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F9037

Formamide

Name: Formamide
Brand: SIGMA

≥99.5% (GC), BioReagent, Molecular Biology

Synonym(s):

Amide C₁, Formic amide

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About This Item

Linear Formula:

HCONH₂

CAS Number:

75-12-7

Molecular Weight:

45.04

UNSPSC Code:

12352111

NACRES:

NA.52

PubChem Substance ID:

24894985

EC Number:

200-842-0

Beilstein/REAXYS Number:

505995

MDL number:

MFCD00007941

Assay:

≥99.5% (GC)

Grade:

Molecular Biology

Technique(s):

electrophoresis: suitable, immunohistochemistry: suitable

Bp:

210 °C (lit.)

Vapor pressure:

0.08 mmHg ( 20 °C), 30 mmHg ( 129 °C)

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Properties

grade

Molecular Biology

Quality Level

200

vapor density

1.55 (vs air)

vapor pressure

0.08 mmHg ( 20 °C), 30 mmHg ( 129 °C)

product line

BioReagent

assay

≥99.5% (GC)

form

liquid

autoignition temp.

932 °F

technique(s)

electrophoresis: suitable, immunohistochemistry: suitable

refractive index

n20/D 1.447 (lit.)

pH

4-10 (20 °C, 200 g/L)

bp

210 °C (lit.)

mp

2-3 °C (lit.)

density

1.134 g/mL at 25 °C (lit.)

suitability

suitable for nucleic acid hybridization

storage temp.

2-8°C

SMILES string

NC=O

InChI

1S/CH3NO/c2-1-3/h1H,(H2,2,3)

InChI key

ZHNUHDYFZUAESO-UHFFFAOYSA-N

Description

General description

Formamide is a clear liquid amide derived from formic acid. Formamide allows for the denaturation and renaturation of nucleic acids at room temperature.

Application

nucleic acid hybridization
cryoprotectant and gel-stabilizer
destabilize nucleic acid duplexes
elimination of secondary structure of nucleic acids
preparation of hybridization buffers for RNA and DNA application

Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.

Formamide is suitable for use in hybridization buffers made using standard recipes. Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.

Other applications include:

Preparation of brain sections of mice for BrdU immunohistochemistry
FISH analysis of HeLa cells.
Preparation of the polyacrylamide gel for analysis of PCR products using denaturing gradient gel electrophoresis(The Merck Index, 12th ed).

Features and Benefits

Deionized for molecular biology applications
Packaged under argon gas in amber bottles to protect from oxidation
Used for DNA renaturation or DNA-RNA hybridization
Reduces thermal stability of double stranded nucleic acids

Other Notes

The product has been deionized for molecular biology use and packaged under argon gas in amber glass bottles to protect it from oxidation.

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pictograms

GHS08

signalword

Danger

hcodes

H351,H360D,H373

pcodes

P202 - P260 - P280 - P308 + P313 - P405 - P501

Hazard Classifications

Carc. 2 - Repr. 1B - STOT RE 2 Oral

target_organs

Blood

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

flash_point_f

305.6 °F

flash_point_c

152 °C

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

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Global Trade Item Number

SKU	GTIN
F9037-100ML	04061837897078