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H2625

Hemoglobin from bovine blood

Name: Hemoglobin from bovine blood
Brand: SIGMA

suitable for protease substrate, substrate powder

Synonym(s):

Bovine hemoglobin, Hb, Methemoglobin

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About This Item

CAS Number:

9008-02-0

UNSPSC Code:

12352202

eCl@ss:

42030116

NACRES:

NA.61

MDL number:

MFCD00131282

Form:

substrate powder

Biological source:

bovine blood

Mol wt:

M_r ~64500

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Properties

biological source

bovine blood

Quality Level

300

form

substrate powder

mol wt

M_r ~64500

technique(s)

activity assay: suitable

solubility

H₂O: soluble 20 mg/mL

suitability

suitable for protease substrate

UniProt accession no.

P01966, P02070, P02081, P06642, P06643

storage temp.

2-8°C

Gene Information

cow ... HBA1(281221), HBB(280813), HBE2(513240), HBE4(513108), HBG(511735), alpha globin(512439)

Description

General description

Hemoglobin is the major component of red blood cells and is responsible for their red color. Its normal concentration in erythrocytes is 34%. Hemoglobin is a globular protein with α and β chains with every 141 and 146 amino acids respectively. It exists as a tetramer with each monomer having heterocyclic porphyrin ring with iron constituting the heme.

Application

Hemoglobin from bovine blood has been used as a substrate in cathepsin D activity assay. It has also been used to measure the activity of acid proteases (pepsin-like) in the stomach extract.

The solubility of α-elastin has been applied to construction of elastin-mimetic biomaterials.

Biochem/physiol Actions

Bovine hemoglobin is used in the production of hemoglobin-vesicles (HbV). It has high thermal stability and high oxygen affinity when compared to human hemoglobin. Bovine hemoglobin interacts with synthetic and azo dyes. Polymerized forms of bovine hemoglobin are used to treat autoimmune hemolytic anemia.

Oxygen transporter, NO scavenger

Oxygen transporter. The Fe²⁺/Fe³⁺ balance is a physiological indicator of blood oxygenation; deoxygenated hemoglobin accessorizes a feedback loop by reducing nitrite to NO, a vasodilator which enhances blood flow to oxygen-deprived tissues.

Preparation Note

Prepared from washed, lysed and dialyzed erythrocytes.

Disclaimer

Since native hemoglobin is readily oxidized in air, these preparations may be predominantly methemoglobin.

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

监管及禁止进口产品

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Protocols

胃蛋白酶的酶法测定（3.4.23.1）

本方法可用于以血红蛋白为底物的胃蛋白酶活性测定。这是一种分光光度速率终止测定法。

Enzymatic Assay of Pepsin (3.4.23.1)

This procedure may be used for determination of Pepsin activity using hemoglobin as the substrate. It is a spectrophotometric stop rate determination.

Proteinase K Enzymatic Assay

Proteinase K activity measured via spectrophotometry using hemoglobin substrate, crucial for enzyme characterization.

Global Trade Item Number

SKU	GTIN
H2625-100G	04061838820259
H2625-1KG	04061826718384
H2625-25G	04061835516070