Invertase Glycoprotein Standard

Name: Invertase Glycoprotein Standard
Brand: SIGMA

BioReagent, from Saccharomyces cerevisiae

Synonym(s):

Invertase from baker’s yeast (S. cerevisiae), β-D-Fructofuranosidase, β-D-Fructofuranoside fructohydrolase, Saccharase

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About This Item

CAS Number:

9001-57-4

UNSPSC Code:

12352202

NACRES:

NA.32

EC Number:

232-615-7

MDL number:

MFCD00131410

EC Number:

3.2.1.26 (BRENDA, IUBMB)

Biological source:

Saccharomyces cerevisiae

Concentration:

≥0.5 mg/vial protein (E1%/280)

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Properties

biological source

Saccharomyces cerevisiae

Quality Level

200

product line

BioReagent

form

lyophilized powder

mol wt

60 kDa

concentration

≥0.5 mg/vial protein (E1%/280)

storage temp.

2-8°C

Description

Application

Invertase Glycoprotein Standard has been used:

to generate N-linked glycan library
as a negative control to study the binding of lectins to high mannose structures
for sample pre-treatment in proteomic analyses to study drug-induced toxic epidermal necrolysis

The Invertase Glycoprotein Standard can be used to demonstrate N-glycosylation using PNGase F with both in-solution and in-gel procedures. The extent of deglycosylation can be assessed by mobility shift on SDS-PAGE gels.

Used in the production of confectionary foods and artificial honey.

Biochem/physiol Actions

Invertase hydrolyzes sucrose into glucose and fructose yielding a colorless product, unlike acid hydrolysis which produces colored products.

Other Notes

Invertase is an enzyme that catalyses the hydrolysis of sucrose into fructose and glucose. Invertase Glycoprotein Standard is the periplasmic (glycosylated form, external invertase) with 50% of its mass as polymannan. Since yeast can provide an alternative system for protein glycosylation that is similar to mammalian systems, periplasmic invertase is often used as a model for the study of the function of oligosaccharides in glycoproteins and for studies on glycoprotein biosynthesis.