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I9516

2-Propanol

≥99.5%, for molecular biology, suitable for RNA extraction, DNA extraction, BioReagent

Synonym(s):

sec-Propyl alcohol, IPA, Isopropanol, Isopropyl alcohol

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About This Item

Linear Formula:
(CH3)2CHOH
CAS Number:
67-63-0
Molecular Weight:
60.10
NACRES:
NA.52
PubChem Substance ID:
24896158
UNSPSC Code:
12352104
EC Number:
200-661-7
MDL number:
MFCD00011674
Beilstein/REAXYS Number:
635639
Assay:
≥99.5%
Grade:
Molecular Biology
Technique(s):
DNA extraction: suitable, RNA extraction: suitable
Bp:
82 °C (lit.)
Vapor pressure:
33 mmHg ( 20 °C), 44 mmHg ( 25 °C)
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Product Name

2-Propanol, BioReagent, ≥99.5%, Molecular Biology

grade

Molecular Biology

vapor density

2.1 (vs air)

vapor pressure

33 mmHg ( 20 °C), 44 mmHg ( 25 °C)

product line

BioReagent

assay

≥99.5%

form

liquid

autoignition temp.

750 °F

expl. lim.

2.0-12.7 %, 93 °C

technique(s)

DNA extraction: suitable, RNA extraction: suitable

impurities

≤0.2% water
water

color

colorless

refractive index

n20/D 1.377 (lit.)

bp

82 °C (lit.)

mp

−89.5 °C (lit.)

solubility

water: soluble (completely)

density

0.785 g/mL at 25 °C (lit.)

suitability

suitable for precipitation of nucleic acids

storage temp.

room temp

SMILES string

CC(C)O

InChI

1S/C3H8O/c1-3(2)4/h3-4H,1-2H3

InChI key

KFZMGEQAYNKOFK-UHFFFAOYSA-N

General description

Isopropanol is a clear, colorless, polar organic solvent commonly used in chemistry and molecular biology laboratories. It will dissolve a wide range of chemicals, and evaporates quickly. This product (2-propanol) is designated as molecular biology grade and is suitable for the precipitation of nucleic acids.

Protocols for the use of isopropanol:

  • DNA precipitation from mammalian tissue, ethidium bromide extraction from DNA samples, and RNA isolation from cells and tissues.
  • Protein crystallization.
  • Isolate retinoids from biological matrices for HPLC and HPLC-MS analysis.

Application

2-Propanol has been used:
  • in DNA and RNA extraction
  • for determination of hydroxyproline levels(The Merck Index, 12th ed)
  • for checking cell viability by MTT assay
  • in microarray hybridization
Suitable for use in DNA precipitation using standard protocols. Also suitable as a solvent for making solutions for molecular biology applications.

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target_organs

Central nervous system

pictograms

FlameExclamation mark
GHS02,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3

Storage Class

3 - Flammable liquids

wgk

WGK 1

flash_point_f

53.6 °F - closed cup

flash_point_c

12.0 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Regulatory Information

危险化学品

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Certificates of Analysis (COA)

Lot/Batch Number
0000157104
SHBP3174
SHBP2153
SHBP0616
BCCG6328

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Protocols

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使用RIP试剂盒进行哺乳动物细胞Ago抗体-RNA免疫沉淀的实验方案和流程

TRI Reagent® 实验方案

TRI试剂是一种单步RNA分离试剂，可同时分离DNA、RNA和蛋白质。本实验方案概述了样品制备方法，以及各种样品提取方法和故障排除方案。

Plasmid DNA Preparation

Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation between Cold Spring Harbor Laboratory Press and our research team.

View All Protocols

Articles

疫苗和药物开发用mRNA合成

了解 mRNA 疫苗如何诱导免疫，以及如何为疫苗免疫原和其他生物制药制备合成 mRNA。寻找合成 mRNA 所需的试剂。

SeqPlex™-I Transcriptome Amplification Kits

SeqPlex™-I WTA kit amplifies RNA for NGS, enabling genomic studies from limited samples.

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F Yang et al.
Journal of molecular biology, 288(3), 403-412 (1999-05-18)
The crystal structure of cyanovirin-N (CV-N), a protein with potent antiviral activity, was solved at 1.5 A resolution by molecular replacement using as the search model the solution structure previously determined by NMR. The crystals belong to the space group
Zsuzsanna Rácz et al.
Nucleic acid therapeutics, 21(3), 215-224 (2011-07-14)
Short-interfering RNAs (siRNAs), key mediators of RNA interference comprise a promising therapeutic tool, although side effects such as interferon (IFN) response are still not perfectly understood. Further, delivery to target organs is a major challenge, possibly associated with side effects
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, E-E (1989)
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Global Trade Item Number

SKUGTIN
I9516-25ML04061837897221
I9516-1L04061841441250
I9516-4L04061841441267
I9516-4X25ML04061837897238
I9516-500ML04061837897245