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L7533

LB Broth with agar (Lennox)

Name: LB Broth with agar (Lennox)
Brand: SIGMA

EZMix powder microbial growth medium

Synonym(s):

LB broth

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About This Item

NACRES:

NA.85

UNSPSC Code:

41106200

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Properties

grade

Molecular Biology

Quality Level

200

sterility

non-sterile

form

powder

composition

Agar, 15 g/L , NaCl, 5 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L

technique(s)

microbiological culture: suitable

application(s)

agriculture
food and beverages
microbiology

storage temp.

room temp

suitability

nonselective for Escherichia coli, nonselective for coliforms

Description

General description

Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.

Application

Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin^®.

Features and Benefits

Lennox EZMix powder with agar provides:

A budget-friendly alternative to pre-poured plates
Granulated, dust-free format for safer handling and faster mixing
Convenient small package to eliminate weighing
Easy scale-up using larger package sizes
Standard formulation

Preparation Note

Add 35.6g powder in 1L water. Heat to boiling while stirring to dissolve powder. Autoclave for 15 minutes at 121C to sterilize. Allow to cool slightly before making additions, such as antibiotics (if desired). Pour into petri dishes and allow to solidify.

1. Suspend 35.6 g in 1 L of distilled water.
2. Heat to boiling while stirring to dissolve all ingredients completely.
3. Autoclave for 15 minutes at 121°C.
To prepare Lennox L Agar: Add 1 g glucose and proceed with preparation instructions as above.
To prepare the medium of Enquist and Sternberg: Aseptically add 10 ml of sterile 1 M magnesium sulfate after autoclaving.

Legal Information

Zeocin is a registered trademark of Cayla Sarl

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Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Protocols

微生物生长实验方案

在从小型培养到发酵级的不同规模下培养大肠杆菌。包括了用于启动起始培养物、悬浮细胞、单一培养物和平板噬菌体M13的实验方案。

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

Competent Cell Protocols

Technical Article on competent cells. Transformation is a process by which some bacteria take up foreign genetic material (naked DNA) from the environment.

Articles

细菌转化实验方案

感受态细胞生长及其转化（吸收DNA）的通用实验方案。

Bacterial Transformation Protocols

General protocols for growth of competent cells and their transformation (uptake of DNA).

Global Trade Item Number

SKU	GTIN
L7533-1KG	04061833965559
L7533-6X500ML	04061833965566