L8757
Lysing Enzymes from Rhizoctonia solani
crude powder
Synonym(s):
Kitalase
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About This Item
UNSPSC Code:
12352204
form
crude powder
storage temp.
2-8°C
Application
Lysing enzymes at a concentration of 1mg/ml can be used for preparation of protoplasts from Rhizoctonia solani.
Biochem/physiol Actions
Main enzymatic acitivity is β−(1-3)-glucanase, also reported to contain protease, pectinase, and amylase activities.
Storage Class
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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Structural characterization of a neutral antitumour β-D-glucan extracted with hot sodium hydroxide from cultured fruit bodies of Grifola frondosa.
Iino, K., et al
Carbohydrate Research, 141(1), 111-119 (1985)
Molecular characterization of a Stagonospora nodorum lipase gene LIP1
Feng, J., et al
Plant Pathology, 60(4), 698-708 (2011)
Tung-Hsen Liu et al.
New biotechnology, 27(1), 64-69 (2009-09-01)
Novozym 234 was the most frequently used enzyme for production of Rhizoctonia solani protoplasts. Since manufacture of this enzyme was discontinued in the late 1990s, a new procedure was developed by testing lytic enzymes from Sigma and by examining factors