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M1302

M-MLV Reverse Transcriptase

Name: M-MLV Reverse Transcriptase
Brand: SIGMA

Moloney Murine Leukemia Virus enzyme & buffer for cDNA synthesis

Synonym(s):

Moloney Murine Leukemia Virus Reverse Transcriptase

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About This Item

CAS Number:

9068-38-6

UNSPSC Code:

12352202

NACRES:

NA.55

MDL number:

MFCD00283754

Biological source:

Porcine intestinal mucosa

Recombinant:

expressed in E. coli

Concentration:

200 units/μL

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Properties

biological source

Porcine intestinal mucosa

recombinant

expressed in E. coli

form

liquid

usage

sufficient for 200 reactions

feature

dNTPs included: no, hotstart: no

concentration

200 units/μL

technique(s)

RT-PCR: suitable

color

colorless

input

purified RNA

shipped in

wet ice

storage temp.

−20°C

Description

General description

Moloney murine leukemia virus (M-MLV ) reverse transcriptase enzyme is isolated from E. coli expressing a portion of the pol gene of M-MLV on a plasmid. MoMLV RT is made up of 671 amino acid residues. It is a DNA polymerase that uses single-stranded RNA, DNA, or an RNA-DNA hybrid (using a primer) to synthesize a complementary DNA strand.

Application

M-MLV Reverse Transcriptase has been used:

for the preparation of cDNA libraries or for first strand cDNA synthesis
for use in a 2-step RT-PCR assay
in quantitative realtime-polymerase chain reaction (RT-qPCR)
in reverse transcription

Features and Benefits

Thermostable reverse transcriptase active at 37 °C.
Can be used to generate first strand cDNA of up to 7 kb.

Packaging

Supplied with 10× M-MLV reverse transcriptase buffer containing DTT.

Preparation Note

The enzyme is purified from Escherichia coli expressing the pol gene of M-MLV on a plasmid.

Other Notes

One unit incorporates 1 nmol of TTP into acid precipitable material in 10 min. at 37 °C using poly(A):oligo dT as a template:primer.

Legal Information

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.

pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Regulatory Information

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Articles

逆转录

分析基因表达的方法之一是测量基因的mRNA浓度。此类分析面临着若干挑战，例如不同转录本之间的半衰期不同、转录时间模式以及mRNA和蛋白质之间缺乏相关性。

Examination of Lentiviral Library Efficiency

Small interfering RNAs (siRNAs) are powerful tools for gene expression knockdown, widely used in molecular biology.

Reverse Transcription

Challenges in gene expression analysis include mRNA stability, temporal transcription patterns, and mRNA-protein correlation, impacting accuracy.

Global Trade Item Number

SKU	GTIN
M1302-40KU	04061835378135