Murashige and Skoog Modified Basal Salt Mixture

Name: Murashige and Skoog Modified Basal Salt Mixture
Brand: SIGMA

powder, suitable for plant cell culture

Synonym(s):

Murashige and Skoog Modified Basal Salt Mixture, w/o Ammonium Nitrate

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About This Item

NACRES:

NA.72

UNSPSC Code:

12352207

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Properties

form

powder

Quality Level

200

technique(s)

cell culture | plant: suitable

application(s)

agriculture

storage temp.

2-8°C

Description

Application

Murashige and Skoog Modified Basal Salt Mixture has been used:

to promote root development for internode stem suckers
to promote shoot growth for the calluses
for low nitrogen (LN) treatment, for the growth of Arabidopsis thaliana

Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and the Kinetin (cytokinin/cell division promoter).

Preparation Note

Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog Salt mixture (M2909) contain the macronutrients and micronutrients of the original classic formulation without ammonium nitrate. It can be combined with M&S vitamins or Gamborg′s vitamins and supplemented with sucrose, agar, auxins (IAA) and cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture.

Without NH₄NO₃
With the macro- and micronutrients described by Murashige and Skoog (1962).

Media Formulation