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M5519

Murashige and Skoog Basal Medium

powder, suitable for plant cell culture

Synonym(s):

MS0 Medium, MSO Medium, MS Basal Medium

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.72
MDL number:
Technical Service
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form

powder

Quality Level

technique(s)

cell culture | plant: suitable

application(s)

agriculture

storage temp.

2-8°C

General description

Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and the Kinetin (cytokinin/cell division promoter).

Application

Murashige and Skoog medium has been used in plant tissue culture medium for plant transformation study.

Preparation Note

Formulated to contain 4.4 grams of powder per liter of medium.
Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog medium (M5519) contains the micronutrients and vitamins of the original classic formulation. It can be supplemented with sucrose, agar, auxins (IAA) and cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture.
With the macro- and micronutrients, and vitamins as described by Murashige and Skoog (1962).

Media Formulation


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Warning

hcodes

Hazard Classifications

Eye Irrit. 2 - Ox. Sol. 3

Storage Class

5.1B - Oxidizing hazardous materials

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

监管及禁止进口产品

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M Guan et al.
Journal of experimental botany, 66(1), 203-212 (2014-10-16)
Nitrogen (N) remobilization from reserves to sinks is essential for seedling establishment and seed production. Cytosolic glutamine synthetase (GS1) is up-regulated during both seed germination and seed filling in plants. However, the specific roles of the individual GS1 isogenes with
Amr R A Kataya et al.
PloS one, 12(7), e0180478-e0180478 (2017-07-06)
Eukaryotic protein phosphatase 4 (PP4) is a PP2A-type protein phosphatase that is part of a conserved complex with regulatory factors PSY2 and PP4R2. Various lines of Arabidopsis thaliana with mutated PP4 subunit genes were constructed to study the so far
Cécile Sulmon et al.
PloS one, 6(11), e26855-e26855 (2011-11-11)
An Arabidopsis thaliana T-DNA insertional mutant was identified and characterized for enhanced tolerance to the singlet-oxygen-generating herbicide atrazine in comparison to wild-type. This enhanced atrazine tolerance mutant was shown to be affected in the promoter structure and in the regulation



Global Trade Item Number

SKUGTIN
M5519-BG04061824042252
M5519-10L04061838259745
M5519-1L04061835568857
M5519-50.1K04061832957388
M5519-50K338204061832957395
M5519-50L04061838259752