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About This Item
NACRES:
NA.52
UNSPSC Code:
41105501
usage
sufficient for 70 purifications
technique(s)
DNA purification: suitable
input
sample(s) (gel slices)
test parameters
sample volume: 3.5 g agarose
storage temp.
15-25°C
General description
The GenElute Gel Extraction Kit is designed for the rapid purification of linear and plasmid DNA fragments from standard or low-melting agarose gels. This kit can also be used to purify DNA from polyacrylamide gels. Typical recovery of DNA is up to 80%. Each column can bind up to 10 μg of DNA from up to a 3.5 g agarose slice.
Application
GenElute™ Gel Extraction Kit has been used for the purification of DNA fragments from agarose gels.
The isolated DNA is suitable for a variety of downstream applications, such as sequencing, PCR, restriction digestion, cloning and labeling.
Biochem/physiol Actions
The GenElute Gel Extraction Kit combines silica-binding technology with the convenience of a spin or vacuum column format. DNA fragments of interest are extracted from slices of an agarose gel and are bound to a silica membrane. Contaminants are removed by a simple spin or vacuum wash. The bound DNA is then eluted.
The purified DNA is suitable for a variety of downstream applications, such as automated DNA sequencing, PCR, restriction digestion, cloning, and labeling.
The purified DNA is suitable for a variety of downstream applications, such as automated DNA sequencing, PCR, restriction digestion, cloning, and labeling.
The GenElute Gel Extraction Kit combines silica-binding technology with the convenience of a spin or vacuum column format. DNA fragments of interest are extracted by solubilizing slices of an agarose gel. The Gel Solubilization Solution dissolves an agarose gel slice from gels run in TBE or TAE buffer. The extracted DNA fragments selectively adsorb onto the silica membrane in the presence of the Gel Solubilization Solution. Contaminants are removed by a simple spin or vacuum wash. Finally, the bound DNA is eluted in Tris buffer.
Features and Benefits
- Bind up to 10 μg of DNA
- Recoveries up to 80%
- Up to 3.5 g can be processed per column
- Compatible with both standard and low-melting agarose in TAE or TBE buffer
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
Kit Components Only
Product No.
Description
- Gel Solubilization Solution
- Elution Solution (10 mM Tris-HCl, pH 9.0)
Kit Components Also Available Separately
Product No.
Description
SDS & Pricing
- Column Preparation Solution
signalword
Danger
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1C - STOT SE 3
target_organs
Respiratory system
supp_hazards
Storage Class
8A - Combustible corrosive hazardous materials
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 3
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Protocols
The GenElute Gel Extraction kit is designed for the rapid purification of 50 bp to 10 kb linear DNA fragments and plasmids from standard or low-melting agarose gels.
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| NA1111-1KT | 04061835573714 |