P5400
Pectinesterase from orange peel
lyophilized powder, ≥150 units/mg protein
Synonym(s):
Pectin methylesterase, Pectin pectylhydrolase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-807-0
MDL number:
Specific activity:
≥150 units/mg protein
form
lyophilized powder
Quality Level
specific activity
≥150 units/mg protein
composition
Protein, 20-50% biuret
greener alternative product characteristics
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
greener alternative category
, Enabling
storage temp.
2-8°C
General description
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been used as enzyme for alternative energy. For more information see the Enzymes for Alternative Energy Research.
Application
The enzyme from Sigma has been used as a standard during the measurement of pectin methylesterase activity in sea buckthorn juices. It has been used in the hydrolysis of cell wall pectins while studying phyllotaxis in Arabidopsis. It has also been used to catalyze pectin hydrolysis (using an ultrasonic measurement device) for monitoring biological processes.
Pectinesterase is used to hydrolyze methyl esters of pectin to pectate and methanol. It contains pectin methylesterase and pectin pectylhydrolase. Product P5400 is from orange peel and comes as a lyophilized powder. It contains (NH4)2SO4 and sodium chloride. It has been used to study the pectin methylesterase gene.
Biochem/physiol Actions
Pectinesterase catalyzes the hydrolysis of the methyl esters of pectin to yield pectate and methanol.
Physical form
Contains (NH4)2SO4 and sodium chloride
Analysis Note
Protein determined by biuret.
Other Notes
One unit will release 1.0 microequivalent of acid from pectin per min at pH 7.5 at 30 °C.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
低风险生物材料
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Kerstin Müller et al.
Plant physiology, 161(1), 305-316 (2012-11-07)
The methylesterification status of cell wall homogalacturonans, mediated through the action of pectin methylesterases (PMEs), influences the biophysical properties of plant cell walls such as elasticity and porosity, important parameters for cell elongation and water uptake. The completion of seed
Sergio T de Freitas et al.
The Plant journal : for cell and molecular biology, 71(5), 824-835 (2012-05-09)
Blossom-end rot (BER) in tomato fruit (Solanum lycopersicum) is believed to be a calcium (Ca²⁺) deficiency disorder, but the mechanisms involved in its development are poorly understood. Our hypothesis is that high expression of pectin methylesterases (PMEs) increases Ca²⁺ bound
Yuan Zhou et al.
Phytochemistry, 76, 46-51 (2012-01-11)
Aluminum (Al) toxicity is one of the most widespread problems for crop production on acid soils, and nitric oxide (NO) is a key signaling molecule involved in the mediation of various biotic and abiotic stresses in plants. Here we found
Global Trade Item Number
| SKU | GTIN |
|---|---|
| P5400-1KU | 04061834391654 |