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Merck
CN

PP0540

GlycoProfile β-Elimination Kit

Sufficient for 24 samples

Synonym(s):

β-Elimination Glycan Kit, Glycan Removal Kit

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About This Item

NACRES:
NA.28
UNSPSC Code:
12352200
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conjugate

(O-linked)

Quality Level

storage temp.

room temp

Application

The GlycoProfile β-Elimination kit contains a novel non-reducing reagent to efficiently and specifically remove O-linked carbohydrates from glycoproteins with minimal protein or glycan destruction. Consequently, additional downstream proteomics and glycomic analyses may be employed.

Preparation Note

The kit includes the reagents and techware for O-deglycosylation of up to 24 samples (200 uL at 1-10 ug/uL of glycoprotein per sample).

Legal Information

GlycoProfile is a trademark of Sigma-Aldrich Co. LLC


Kit Components Only

Product No.
Description

  • Sodium hydroxide solution, 5.0 M 60 μL

  • Microcon centrifugal filter unit, YM-10 membrane, NMWCO 10 kDa 24 ea

Kit Components Also Available Separately

Product No.
Description
SDS & Pricing

  • Centrifugal Filter Unit
    SDS

pictograms

FlameCorrosion

signalword

Danger

Hazard Classifications

Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1A

Storage Class

3 - Flammable liquids

flash_point_f

82.0 °F - closed cup

flash_point_c

27.79 °C - closed cup

Regulatory Information

危险化学品

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Protocols

Explore mass spectrometry analysis of glycans for glycomic & glycoproteomic neutral & acidic glycan analysis. See a general mass spec glycan analysis procedure.

Articles

Our GlycoProfile Beta-Elimination Kit enables comprehensive O-glycoproteomics research, overcoming challenges in complete O-deglycosylation.

Find deglycosylation kits for your research. Review the different features of deglycosylation kits for glycoproteins, enzymatic proteins, and native proteins.

Learn about O-linked glycan strategies, O-glycosidase actions, how to remove sialic acid residues, β-Elimination, and O-glycan modifications.

Related Content

Glycobiology and Glycoproteomics Brochure


Prediction of glycoprotein secondary structure using ATR-FTIR
Lewis, S.P., et al.
Vibrational Spectroscopy, 69, 21-29 (2013)



Global Trade Item Number

SKUGTIN
PP0540-1KT04061836686932