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R7884

Ribonuclease B from bovine pancreas

Name: Ribonuclease B from bovine pancreas
Brand: SIGMA

BioReagent, ≥50 Kunitz units/mg protein, ≥80% (SDS-PAGE)

Synonym(s):

RNase B

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About This Item

CAS Number:

9001-99-4

EC Number:

3.1.27.5 (BRENDA, IUBMB)

UNSPSC Code:

12352204

EC Number:

232-646-6

NACRES:

NA.32

MDL number:

MFCD00132184

Specific activity:

≥50 Kunitz units/mg protein

Assay:

≥80% (SDS-PAGE)

Biological source:

bovine pancreas

Concentration:

≥60%

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Properties

Quality Level

200

biological source

bovine pancreas

product line

BioReagent

assay

≥80% (SDS-PAGE)

form

powder

specific activity

≥50 Kunitz units/mg protein

concentration

≥60%

technique(s)

cell based assay: suitable

suitability

suitable for molecular biology

application(s)

cell analysis

foreign activity

protease ≤0.001 units/mg solid

storage temp.

−20°C

SMILES string

[nH]1cncc1CC(NC(=O)CCN)C(=O)O

InChI

1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)

InChI key

CQOVPNPJLQNMDC-UHFFFAOYSA-N

Description

Application

Ribonuclease B from bovine pancreas is used in the digestion of RNA during cell cycle platform analysis.

Biochem/physiol Actions

Native RNase BS generated by subtilisin digestion of native RNase B comprising of amino acid residues 21-124 of RNase B, is sensitive to PNGase F digestion. Intramolecular N-glycans of bovine pancreatic RNase B function like chaperone. RNase B is found to be much faster than RNase A, while RNase A is liable to aggregate during regeneration. The stimulatory effect of Asn-oligosaccharide (which corresponds to the most predominant sugar chain of RNase B) reveals that the N-glycans of RNase B facilitates the transformation of bulky intermediates into folded, compact species.

Packaging

Package size based on protein content

Preparation Note

Purified by affinity chromatography

pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

低风险生物材料

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Articles

Rapid Glycan Prep & Analysis by LC-MS Workflow

PNGase Fast denaturing buffer and enzyme provide results similar to a conventional 20-hour protocol, reducing workflow time to about 1 hour.

The structural basis of the difference in sensitivity for PNGase F in the de-N-glycosylation of the native bovine pancreatic ribonucleases B and BS.

Véronique Blanchard et al.

Biochemistry, 47(11), 3435-3446 (2008-02-26)

In glycoanalysis protocols, N-glycans from glycoproteins are most frequently released with peptide- N (4)-( N-acetyl-beta-glucosaminyl)asparagine amidase F (PNGase F). As the enzyme is an amidase, it cleaves the NH-CO linkage between the Asn side chain and the Asn-bound GlcNAc residue.

A chaperone-like function of intramolecular high-mannose chains in the oxidative refolding of bovine pancreatic RNase B.

H Yamaguchi et al.

Journal of biochemistry, 120(3), 474-477 (1996-09-01)

This paper describes a chaperone-like function of the intramolecular N-glycans of bovine pancreatic RNase B. We studied air-oxidative regeneration from reductively denatured species of RNase B and its nonglycosylated form, RNase A. RNase B was reactivated much faster than RNase

Glycosylation States on Intact Proteins Determined by NMR Spectroscopy.

Audra A Hargett et al.

Molecules (Basel, Switzerland), 26(14) (2021-07-25)

Protein glycosylation is important in many organisms for proper protein folding, signaling, cell adhesion, protein-protein interactions, and immune responses. Thus, effectively determining the extent of glycosylation in glycoprotein therapeutics is crucial. Up to now, characterizing protein glycosylation has been carried

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Global Trade Item Number

SKU	GTIN
R7884-500UG	04061836807344
R7884-100MG	04061836801892
R7884-5MG	04061833290323