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About This Item
NACRES:
NA.55
UNSPSC Code:
41121800
Quality Level
technique(s)
whole genome amplification: suitable
dilution
(WGA)
input
purified DNA
shipped in
wet ice
storage temp.
−20°C
General description
SeqPlex™ Enhanced DNA Amplification Kit for whole genome amplification (WGA) is designed to facilitate next-generation sequencing (NGS) from extremely small quantities or from degraded/highly fragmented DNA. The yields from chromatin immunoprecipitation (ChIP) or formalin-fixed paraffin-embedded tissue samples (FFPE) are often less than required for successful next generation sequencing library preparation. The SeqPlex kit allows the user to pre-amplify these and other small quantity/highly fragmented DNA samples for input into a NGS workflow. This kit is an extension of the WGA product line and has been developed to integrate into the Illumina®, SOLiD™, or 454 sequencing workflows.
Application
SeqPlex™ DNA Amplification Kit has been used for whole genome amplification.
Features and Benefits
- Random priming technology amplifies fragmented DNA such as ChIP or FFPE
- Facilitates sequencing from as little as 100 pg of ChIP DNA
- Enhanced primers for complete genome coverage, minimal sequence bias, primer removal, and amplicon size ideal for next gen sequencing
- Compatible with Illumina®, SOLiD™, or 454 library prep for next generation sequencing
Other Notes
SEQXE-500RXN is manufactured on-demand. Contact technical services at techserv@sial.com for more information.
Legal Information
Illumina is a registered trademark of Illumina, Inc.
SOLiD is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
SeqPlex is a trademark of Sigma-Aldrich Co. LLC
Kit Components Also Available Separately
Product No.
Description
SDS & Pricing
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 3
Regulatory Information
常规特殊物品
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Protocols
面向全基因组扩增(WGA)的SeqPlexDNA扩增试剂盒(SEQXE)专用于对极小量或降解/高度片段化的DNA进行下一代测序(NGS)。
Sigma Imprint染色质免疫沉淀试剂盒利用检测板系统,可实现快速的高通量ChIP检测
SeqPlex DNA Amplification Kit enables NGS from small or degraded DNA quantities for whole genome amplification.
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| SEQXE-10RXN | 04061837018138 |
| SEQXE-500RXN | 04061837018145 |
| SEQXE-50RXN | 04061837018176 |
