Yeast Synthetic Drop-out Medium Supplements

The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.

1.92 g will supplement 1 liter of medium.

Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids.

The supplements contain all of the following components except as indicated:
Amino acids: All standard amino acids except for tryptophan. All the amino acids are present at a concentration of 76 mg/L except for leucine, which is present at a concentration of 380 mg/L.
Other nutrients: Adenine (18 mg/L), inositol (76 mg/L), p-aminobenzoic acid (8 mg/L), uracil (76 mg/L).