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53567-U

Ascentis^® Express 90 Å F5 (2.7 μm) HPLC Columns

Name: Ascentis<SUP>&#174;</SUP> Express 90 Å F5 (2.7 μm) HPLC Columns
Brand: SUPELCO

L × I.D. 5 cm × 2.1 mm, HPLC Column

Synonym(s):

Core-shell (SPP) Fused Core PFP HPLC column

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About This Item

UNSPSC Code:

41115700

eCl@ss:

32110501

NACRES:

SB.52

L × i.d.:

5 cm × 2.1 mm

Particle size:

2.7 μm

Matrix active group:

PFP (pentafluorophenyl) phase

Pore size:

90 Å pore size

Matrix:

Fused-Core particle platform, superficially porous particle

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Properties

Product Name

Ascentis^® Express F5, 2.7 μm HPLC Column, 2.7 μm particle size, L × I.D. 5 cm × 2.1 mm

material

stainless steel column

Quality Level

100

agency

suitable for USP L43

product line

Ascentis^®

feature

endcapped

manufacturer/tradename

Ascentis^®

packaging

1 ea of

parameter

60 °C temp. range, 600 bar max. pressure (9000 psi)

technique(s)

HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable

L × I.D.

5 cm × 2.1 mm

surface area

135 m²/g

impurities

<5 ppm metals

matrix

Fused-Core particle platform, superficially porous particle

matrix active group

PFP (pentafluorophenyl) phase

particle size

2.7 μm

pore size

90 Å pore size

operating pH

2-8

application(s)

food and beverages

separation technique

reversed phase

Description

General description

The pentafluorophenylpropyl stationary phase of Ascentis Express F5 provides a stable reversed phase packing with electron-deficient phenyl rings due to the presence of electronegative fluorines.In addition to forming pi-pi and mildly steric interactions, F5 phases also retain compounds by polar interactions. Ascentis Express F5 can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Protocols

Fast and Accurate LC-MS Analysis of Vitamin D Metabolites Using Ascentis® Express F5 HPLC Columns

Fast and Accurate LC-MS Analysis of Vitamin D Metabolites, Using Ascentis® Express F5 HPLC Columns from Supelco®

Articles

Ascentis® Express F5 (Pentafluorophenyl) U/HPLC Columns

Enhanced selectivity for stereoisomers and closely related compounds with alternate selectivity from C18 phases for basic, acidic, or neutral compounds

Impact of Mobile Phase Additives on LC-MS Sensitivity, Demonstrated using Spice Cannabinoids

This study demonstrates the impact of various mobile phase modifiers on the separation; formate modifiers outperform acetate in terms of MS signals (or sensitivity) and chromatographic resolution.

Two Distinct Sample Prep Approaches to Overcome Matrix Effect in LC/MS of Serum or Plasma Samples

This article highlights the impact that sample matrix effects can have on LC/MS response and discusses two novel approaches to reduce it.

Global Trade Item Number

SKU	GTIN
53567-U	04061838531537