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53599-U

Ascentis® Express 90 Å F5 (2.7 μm) HPLC Columns

L × I.D. 5 mm × 4.6 mm, HPLC Guard Cartridge, pkg of 3 ea

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52
L × i.d.:
5 mm × 4.6 mm
Particle size:
2.7 μm
Matrix active group:
PFP (pentafluorophenyl) phase
Pore size:
90 Å
Matrix:
superficially porous particle
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Product Name

Ascentis® Express F5, 2.7 μm Guard Cartridge, 2.7 μm particle size, L × I.D. 5 mm × 4.6 mm, pkg of 3 ea

material

stainless steel column

Quality Level

100

agency

suitable for USP L43

product line

Ascentis®

feature

endcapped

packaging

pkg of 3 ea

technique(s)

HPLC: suitable, LC/MS: suitable

L × I.D.

5 mm × 4.6 mm

surface area

135 m2/g

matrix

superficially porous particle

matrix active group

PFP (pentafluorophenyl) phase

particle size

2.7 μm

pore size

90 Å

operating pH

2-8

application(s)

food and beverages

separation technique

reversed phase

General description

Ascentis Express Guard Columns provide physical (filtration) and chemical protection for costly analytical columns without compromising the very high performance of Ascentis Express columns. These Ascentis Express guard columns are capable of continuous use at pressures up to 9000 psi (600 bar) with only hand-tightening. Guard cartridges are easily replaced without removing the guard column holder from the flow path. The cartridges are packed with Ascentis Express Fused-core® particles. Order guard column holder separately.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

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Protocols

Fast and Accurate LC-MS Analysis of Vitamin D Metabolites Using Ascentis® Express F5 HPLC Columns

Fast and Accurate LC-MS Analysis of Vitamin D Metabolites, Using Ascentis® Express F5 HPLC Columns from Supelco®

Articles

Ascentis® Express F5 (Pentafluorophenyl) U/HPLC Columns

Enhanced selectivity for stereoisomers and closely related compounds with alternate selectivity from C18 phases for basic, acidic, or neutral compounds

Iltaf Shah et al.
The Journal of steroid biochemistry and molecular biology, 180, 118-128 (2018-02-13)
Research shows that immunoassay techniques are not the best choice for the estimation of vitamin D in human blood samples. The main reasons are that some immunoassays are not able to distinguish between 25-OHD3 and 25-OHD2 vitamin D metabolites. Furthermore

Global Trade Item Number

SKUGTIN
53599-U04061838531704