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53811-U

Ascentis^® Express C18 (2.7 μm) HPLC Column

Name: Ascentis<SUP>®</SUP> Express C18 (2.7 μm) HPLC Column
Brand: SUPELCO

L × I.D. 5 cm × 3 mm, pk of 1 ea

Synonym(s):

Core-shell (SPP) Fused Core C18 HPLC column

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About This Item

UNSPSC Code:

41115700

NACRES:

SB.52

eCl@ss:

32110501

L × i.d.:

5 cm × 3 mm

Particle size:

2.7 μm

Matrix active group:

C18 (octadecyl) phase

Pore size:

90 Å

Matrix:

Fused-Core particle platform, superficially porous particle

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Properties

material

stainless steel column

Quality Level

100

agency

suitable for USP L1

product line

Ascentis^®

feature

endcapped

packaging

pk of 1 ea

manufacturer/tradename

Ascentis^®

parameter

60 °C temp. range, 600 bar max. pressure (9000 psi)

technique(s)

HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable

L × I.D.

5 cm × 3 mm

surface area

135 m²/g

impurities

<5 ppm metals

matrix

Fused-Core particle platform, superficially porous particle

matrix active group

C18 (octadecyl) phase

particle size

2.7 μm

pore size

90 Å

operating pH

2-9

application(s)

food and beverages

separation technique

reversed phase

Description

General description

Ascentis Express HPLC columns, through the use of Fused-Core^® particle technology, can provide you with both the high speed and high efficiencies of sub-2 μm particles while maintaining lower backpressures. The combination of high efficiency and low backpressure benefits UPLC^® (or other ultra high pressure system) users, as well as conventional HPLC users.
Visit the Ascentis Express home page for more information on this new column technology.

Application

HPLC Analysis of Capsaicins in Chili Powder on Ascentis^® Express C18, 2.7 μm
UHPLC/MS Analysis of Corn Oil on Ascentis^® Express C30 Compared to Ascentis^® Express C18
UHPLC Analysis of Paracetamol and Impurities on Ascentis^® Express C18, 2.7 μm
HPLC Analysis of Parabens on Ascentis^®Express C18, 2.7 μm

Legal Information

Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

UPLC is a registered trademark of Waters

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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pictograms

GHS07

signalword

Warning

hcodes

H317

pcodes

P280

Hazard Classifications

Skin Sens. 1

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Articles

Newly Developed Porous Graphitic Carbon in U/HPLC

Polar graphitic carbon enables UHPLC separation of highly polar pesticides, herbicides, and Vitamin D metabolites, advancing liquid chromatography capabilities.

Derivatization and Separation of Aliphatic Amines

Derivatization of Aliphatic Amines - Results from the Supelco Ascentis Express column show higher peak resolution, and improved peak shapes than typical chromatograms.

The Carbon Enigma: Material Fundamentals and Retention Properties for Porous Graphitic Carbon (PGC) Stationary Phases

A shortened review of fundamentals of Porous Graphitic Carbon (PGC) materials. What they are and how do they work as a stationary phase material in HPLC?.

Development of a rapid and sensitive method for the determination of aluminum by reverse-phase high-performance liquid chromatography using a fluorescence detector.

Heena et al.

Journal of chromatographic science, 53(5), 800-806 (2014-10-18)

This study represents a new analytical high-performance liquid chromatography-fluorescence detector method for the determination of Al(III) as Al(III) complex with 8-hydroxyquinoline-5-sulfonic acid in a tap water sample and a coke sample. A micellar liquid chromatographic method is proposed for the

High-Throughput Serum 25-Hydroxy Vitamin D Testing with Automated Sample Preparation.

Judy Stone

Methods in molecular biology (Clifton, N.J.), 1378, 301-320 (2015-11-26)

Serum from bar-coded tubes, and then internal standard, are pipetted to 96-well plates with an 8-channel automated liquid handler (ALH). The first precipitation reagent (methanol:ZnSO4) is added and mixed with the 8-channel ALH. A second protein precipitating agent, 1 %

Bioequivalance and pharmacokinetic study of febuxostat in human plasma by using LC-MS/MS with liquid liquid extraction method.

Babu Rao Chandu et al.

SpringerPlus, 2(1), 194-194 (2013-06-07)

A bioequivalence study was proved of generic Febuxostat 80 mg tablets (T) in healthy volunteers.For this purpose, Authors developed a simple, sensitive, selective, rapid, rugged and reproducible liquid chromatography-tandem mass spectrometry method for the quantification of Febuxostat (FB) in human

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Global Trade Item Number

SKU	GTIN
53811-U	04061838534217