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53822-U

Ascentis® Express C18 (2.7 μm) HPLC Column

L × I.D. 5 cm × 2.1 mm, pk of 1 ea

Synonym(s):

Core-shell (SPP) Fused Core C18 HPLC column

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52
eCl@ss:
32110501
L × i.d.:
5 cm × 2.1 mm
Particle size:
2.7 μm
Matrix active group:
C18 (octadecyl) phase
Pore size:
90 Å
Matrix:
Fused-Core particle platform, superficially porous particle
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material

stainless steel column

Quality Level

100

agency

suitable for USP L1

product line

Ascentis®

feature

endcapped

packaging

pk of 1 ea

manufacturer/tradename

Ascentis®

parameter

60 °C temp. range, 600 bar max. pressure (9000 psi)

technique(s)

HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable

L × I.D.

5 cm × 2.1 mm

surface area

135 m2/g

impurities

<5 ppm metals

matrix

Fused-Core particle platform, superficially porous particle

matrix active group

C18 (octadecyl) phase

particle size

2.7 μm

pore size

90 Å

operating pH

2-9

application(s)

food and beverages

separation technique

reversed phase

General description

Ascentis Express HPLC columns, through the use of Fused-Core® particle technology, can provide you with both the high speed and high efficiencies of sub-2 μm particles while maintaining lower backpressures. The combination of high efficiency and low backpressure benefits UPLC® (or other ultra high pressure system) users, as well as conventional HPLC users.
Visit the Ascentis Express home page for more information on this new column technology.

Application

HPLC Analysis of Capsaicins in Chili Powder on Ascentis® Express C18, 2.7 μm
UHPLC/MS Analysis of Corn Oil on Ascentis® Express C30 Compared to Ascentis® Express C18
UHPLC Analysis of Paracetamol and Impurities on Ascentis® Express C18, 2.7 μm
HPLC Analysis of Parabens on Ascentis®Express C18, 2.7 μm

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.
UPLC is a registered trademark of Waters

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pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

pcodes

P280

Hazard Classifications

Skin Sens. 1

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
USMD010430
USMD010429
USMD010428
USMD010427
USMD010426

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Protocols

Fast LC-MS-MS Analysis of 25-Hydroxyvitamin D2 and 25-Hydroxyvitamin D3

We presents a robust method for analyzing 25-hydroxyvitamin D3 and 25-hydroxyvitamin D2 in serum using a multiplexed LC-MS-MS system.

Articles

过滤在(U)HPLC和LC-MS性能优化中的重要性

了解过滤器选择以及样品和流动相过滤如何影响HPLC反压和柱寿命。

Derivatization Agents for LC/MS – An Improved Detection of Estradiol with ESI-MS

Improved LC/MS Detection of Estradiol using 4-(Dimethylamino) benzoyl chloride (DMABC) derivatization agent relative to dansyl chloride.

Derivatization and Separation of Aliphatic Amines

Derivatization of Aliphatic Amines - Results from the Supelco Ascentis Express column show higher peak resolution, and improved peak shapes than typical chromatograms.

View All Articles
Heena et al.
Journal of chromatographic science, 53(5), 800-806 (2014-10-18)
This study represents a new analytical high-performance liquid chromatography-fluorescence detector method for the determination of Al(III) as Al(III) complex with 8-hydroxyquinoline-5-sulfonic acid in a tap water sample and a coke sample. A micellar liquid chromatographic method is proposed for the
Judy Stone
Methods in molecular biology (Clifton, N.J.), 1378, 301-320 (2015-11-26)
Serum from bar-coded tubes, and then internal standard, are pipetted to 96-well plates with an 8-channel automated liquid handler (ALH). The first precipitation reagent (methanol:ZnSO4) is added and mixed with the 8-channel ALH. A second protein precipitating agent, 1 %
Babu Rao Chandu et al.
SpringerPlus, 2(1), 194-194 (2013-06-07)
A bioequivalence study was proved of generic Febuxostat 80 mg tablets (T) in healthy volunteers.For this purpose, Authors developed a simple, sensitive, selective, rapid, rugged and reproducible liquid chromatography-tandem mass spectrometry method for the quantification of Febuxostat (FB) in human
View All Related Papers

Global Trade Item Number

SKUGTIN
53822-U04061838534279