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53829-U

Ascentis® Express C18 (2.7 μm) HPLC Column

L × I.D. 15 cm × 4.6 mm, pk of 1 ea

Synonym(s):

Core-shell (SPP) Fused Core C18 HPLC column

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52
eCl@ss:
32110501
L × i.d.:
15 cm × 4.6 mm
Particle size:
2.7 μm
Matrix active group:
C18 (octadecyl) phase
Pore size:
90 Å
Matrix:
Fused-Core particle platform, superficially porous particle
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material

stainless steel column

Quality Level

100

agency

suitable for USP L1

product line

Ascentis®

feature

endcapped

packaging

pk of 1 ea

manufacturer/tradename

Ascentis®

parameter

60 °C temp. range, 600 bar max. pressure (9000 psi)

technique(s)

HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable

L × I.D.

15 cm × 4.6 mm

surface area

135 m2/g

impurities

<5 ppm metals

matrix

Fused-Core particle platform, superficially porous particle

matrix active group

C18 (octadecyl) phase

particle size

2.7 μm

pore size

90 Å

operating pH

2-9

application(s)

food and beverages

separation technique

reversed phase

General description

Ascentis Express HPLC columns, through the use of Fused-Core® particle technology, can provide you with both the high speed and high efficiencies of sub-2 μm particles while maintaining lower backpressures. The combination of high efficiency and low backpressure benefits UPLC® (or other ultra high pressure system) users, as well as conventional HPLC users.
Visit the Ascentis Express home page for more information on this new column technology.

Application

HPLC Analysis of Capsaicins in Chili Powder on Ascentis® Express C18, 2.7 μm
UHPLC/MS Analysis of Corn Oil on Ascentis® Express C30 Compared to Ascentis® Express C18
UHPLC Analysis of Paracetamol and Impurities on Ascentis® Express C18, 2.7 μm
HPLC Analysis of Parabens on Ascentis®Express C18, 2.7 μm

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.
UPLC is a registered trademark of Waters

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Certificates of Analysis (COA)

Lot/Batch Number
USHW026548
USHW026547
USHW026546
USHW026545
USHW026544

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Protocols

HPLC Separation of Bile Acids with Ascentis Express C18

Investigate bile acid roles in gut hormone profiles and glycemic control, vital for clinical labs exploring potential mechanisms.

Lopinavir HPLC Assay Method

Lopinavir assay setup saves time and improves efficiency with shorter columns, meeting European pharmacopoeia guidelines.

US EPA Method 632: HPLC Analysis of Pesticides on Ascentis® C18

-methylcarbamate 10 μg/mL; Diuron; Propham; Siduron; Methiocarb, analytical standard; Linuron 10 μg/mL; Swep 10 μg/mL; Chlorpropham 10 μg/mL; Barban; Neburon

Articles

HPLC Separation: Ginsenosides from American Ginseng

Optimize HPLC method for ginsenoside separation using a mixture, applying it to American Ginseng root, with conditions and chromatograms shown.

Derivatization and Separation of Aliphatic Amines

Derivatization of Aliphatic Amines - Results from the Supelco Ascentis Express column show higher peak resolution, and improved peak shapes than typical chromatograms.

Improving USP Lansoprazole Analysis with HPLC

USP methods continuously revised for modernization and improved results in drug testing.

Related Content

Bile Acids Kit for LC-MS

Bile Acids (BA) are synthesized in the liver and play important roles in cholesterol homeostasis, absorption of vitamins and lipids, and various key metabolic processes.

Nitrosamines in Pharmaceuticals

Accurately detect and quantify trace nitrosamines (NDMA, NDEA, NEIPA, NDIPA, NDBA, etc.) in pharmaceutical drugs using our complete product portfolio and application guides for LC-MS and GC-MS. Order high-quality reference standards, columns, filters & more.

Assay of Ibuprofen Esters & Impurities

Ibuprofen and impurity separation using Ascentis Express 90Å C18 column with excellent peak shape and low quantification limits.

Heena et al.
Journal of chromatographic science, 53(5), 800-806 (2014-10-18)
This study represents a new analytical high-performance liquid chromatography-fluorescence detector method for the determination of Al(III) as Al(III) complex with 8-hydroxyquinoline-5-sulfonic acid in a tap water sample and a coke sample. A micellar liquid chromatographic method is proposed for the
Judy Stone
Methods in molecular biology (Clifton, N.J.), 1378, 301-320 (2015-11-26)
Serum from bar-coded tubes, and then internal standard, are pipetted to 96-well plates with an 8-channel automated liquid handler (ALH). The first precipitation reagent (methanol:ZnSO4) is added and mixed with the 8-channel ALH. A second protein precipitating agent, 1 %
Babu Rao Chandu et al.
SpringerPlus, 2(1), 194-194 (2013-06-07)
A bioequivalence study was proved of generic Febuxostat 80 mg tablets (T) in healthy volunteers.For this purpose, Authors developed a simple, sensitive, selective, rapid, rugged and reproducible liquid chromatography-tandem mass spectrometry method for the quantification of Febuxostat (FB) in human
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Global Trade Item Number

SKUGTIN
53829-U04061838534323