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581512-U

Ascentis® Si (5 µm) HPLC Columns

L × I.D. 15 cm × 4.6 mm, HPLC Column

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52
L × i.d.:
15 cm × 4.6 mm
Particle size:
5 μm
Matrix active group:
silica phase
Pore size:
100 Å
Matrix:
fully porous particle, silica gel high purity, spherical
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Product Name

Ascentis® Si HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm

material

stainless steel column

Quality Level

100

agency

suitable for USP L3

product line

Ascentis®

feature

endcapped: no

manufacturer/tradename

Ascentis®

packaging

1 ea of

parameter

≤70 °C temp. range, 400 bar pressure (5801 psi)

technique(s)

HPLC: suitable, LC/MS: suitable

L × I.D.

15 cm × 4.6 mm

surface area

450 m2/g

impurities

<5 ppm metals

matrix

fully porous particle, silica gel high purity, spherical

matrix active group

silica phase

particle size

5 μm

pore size

100 Å

operating pH range

2-6

application(s)

food and beverages

separation technique

hydrophilic interaction (HILIC), normal phase

General description

The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.

The Ascentis Si is a high loading capacity silica with excellent peak shape. The Ascentis Si performs in both normal-phase and HILIC/ANP (aqueous normal phase) mode.

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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Certificates of Analysis (COA)

Lot/Batch Number
275720-04
275720-03
275720-02
275720-01
275623-04

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Protocols

HPLC Analysis of Fat Soluble Vitamins by Normal Phase Chromatography on Ascentis® Si

Separation of (±)-α-Tocopherol, analytical standard; Menadione (K3), analytical standard; (+)-γ-Tocopherol, analytical standard; Cholecalciferol (D3), analytical standard

Polar Compound Retention using Aqueous Normal-Phase (ANP/HILIC) Chromatography

Polar Compound Retention using Aqueous Normal-Phase (ANP/HILIC) Chromatography

Paweł Kubica et al.
Journal of chromatography. A, 1289, 13-18 (2013-04-04)
Two analytical procedures are proposed where HILIC and RPLC techniques are coupled with tandem mass spectrometry detection for rapid determination of trace amounts of nicotine in zero-level liquids for electronic cigarettes. Samples are prepared on the basis of the approach
Szabolcs Fekete et al.
Journal of pharmaceutical and biomedical analysis, 50(5), 703-709 (2009-06-30)
The performance of a narrow bore silica based monolith column (5 cm x 2 mm) was compared to 5 cm long narrow bore (internal diameter < or = 2.1 mm) columns, packed with shell particles (2.7 microm) and totally porous
Szabolcs Fekete et al.
Journal of pharmaceutical and biomedical analysis, 49(1), 64-71 (2008-11-29)
The performance of 5 cm long columns packed with shell particles was compared to totally porous sub-2 microm particles in gradient and isocratic elution separations of hormones (dienogest, finasteride, gestodene, levonorgestrel, estradiol, ethinylestradiol, noretistherone acetate, bicalutamide and tibolone). Peak capacities
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Global Trade Item Number

SKUGTIN
581512-U04061838549259