- Proximity Ligation Assay for Detection of R-Loop Complexes upon DNA Damage.
Proximity Ligation Assay for Detection of R-Loop Complexes upon DNA Damage.
Methods in molecular biology (Clifton, N.J.) (2022-06-16)
Adele Alagia, Ruth F Ketley, Monika Gullerova
PMID35704199
ABSTRACT
In situ Proximity Ligation Assay (PLA ) can be used to detect the close proximity (less than ~40 nm) of two biological molecules of interest in cells. Here we report the application of this method for the specific detection of R-loop interacting proteins and RNA modifications in close proximity to R-loops in non-damage and ionizing radiation (IR) induced DNA damage conditions.
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