{"metaDataObj":{"isNoIndex":false,"isNoFollow":false,"keywords":["Analytical chemistry","Protocol","Large molecule HPLC"],"title":"HPLC Analysis of PEG g006200","seoSchemas":[{"@context":"https://schema.org","@type":"BreadcrumbList","itemListElement":[{"@type":"ListItem","position":1,"name":"Large Molecule HPLC","item":"%%HOMEPAGE_URL%%/CN/en/applications/analytical-chemistry/liquid-chromatography/large-molecule-hplc"},{"@type":"ListItem","position":2,"name":"HPLC Analysis of PEG on Two Zenix^® SEC-300 Columns in Tandem"}]}],"description":"HPLC Analysis of PEG on Two Zenix® SEC-300 Columns in Tandem","pageType":"Protocol"},"title":"HPLC Analysis of PEG g006200","lastModifiedDate":1645984161265,"templateName":"technical-article---protocol-page-template","designPath":"/libs/settings/wcm/designs/default","cssClassNames":"page basicpage","language":"en",":type":"cms-commons/components/structure/page",":itemsOrder":["root"],":items":{"root":{"columnCount":12,"columnClassNames":{"rightrailresponsivecontainer":"aem-GridColumn aem-GridColumn--default--3 aem-GridColumn--offset--default--0 aem-GridColumn--default--none","footer":"aem-GridColumn aem-GridColumn--default--12","utilityresponsivecontainer":"aem-GridColumn aem-GridColumn--default--3 aem-GridColumn--offset--default--0 aem-GridColumn--default--none","bodyresponsivecontainer":"aem-GridColumn aem-GridColumn--default--9 aem-GridColumn--offset--default--0 aem-GridColumn--default--none","topresponsivecontainer":"aem-GridColumn aem-GridColumn--default--9 aem-GridColumn--offset--default--0 aem-GridColumn--default--none"},"gridClassNames":"aem-Grid aem-Grid--12 aem-Grid--default--12","allowedComponents":{"applicable":false,"components":[{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/aboutus","title":"About Us Text"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/accordion","title":"Accordion"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/audio","title":"Audio"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/author","title":"Author"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/breadcrumb","title":"Breadcrumbs - Page Path"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/button","title":"Button"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/card","title":"Card"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/carousel","title":"Carousel"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/categorysearchresults","title":"Category Search Results"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/cftext","title":"CF Text"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/contact","title":"Contact Component"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/container","title":"Container component"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/dealerlicense","title":"Dealer License"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/dynamicproducttable","title":"Dynamic Product Table"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/event-sidebar","title":"Event Sidebar"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/eventlisting","title":"Event Listing"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/experiencefragment","title":"Experience Fragment"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/featuretiles","title":"Feature Tiles"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/footer","title":"Footer"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/formAssembly","title":"Form Assembly"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/gatedbutton","title":"Gated Button"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/gatedcontent","title":"Gated Content"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/globalfooter","title":"Global Footer"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/header","title":"Header"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/herocomponent","title":"Hero Component"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/workflows/horizontal","title":"Workflow Horizontal"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/horizontaltab","title":"Tabbed Horizontal Navigation"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/image","title":"Image"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/leftnavigation","title":"Navigation Model"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/locationmap","title":"Map"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/pressreleasegrid","title":"Related Press releases"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/pressreleaselisting","title":"Press release listing"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/presstext","title":"Region Text"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/primarycontent","title":"Primary Content"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/printsocialsharing","title":"Print Social sharing"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/promobanner","title":"Promo Banner"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/rawhtml","title":"Raw HTML"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/recommendation","title":"Recommendation"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/reference","title":"Reference"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/relatedContentCarousel","title":"Related Content Carousel"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/relatedcontentgrid","title":"Related Content Grid"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/relatedcontentlinks","title":"Related Content Links"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/relatedproductresources","title":"Related Product Resources"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/relatedproducts","title":"Related Products Carousel"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/relatedproductsrightrail","title":"Related Products Right Rail"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/relatedwebinar","title":"Related Webinar"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/sectiondivider","title":"Section Divider"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/sitecontentsearch","title":"Site Content Search"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/speakerpage","title":"Speaker Page"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/speakers-list","title":"Speakers List"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/table","title":"Static Table"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/tagbasedbreadcrumb","title":"Breadcrumbs - Tag Based"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/text","title":"Text"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/title","title":"Title"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/topnavigation","title":"Top Navigation"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/upcomingeventsgrid","title":"Upcoming Events"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/workflows/vertical","title":"Workflow Vertical"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/video","title":"Video"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/webinarlisting","title":"Webinar listing"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/webinarsidebar","title":"Webinar Sidebar"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/cms-commons/components/content/worldwideofficelisting","title":"Worldwide Office Listing"}]},":itemsOrder":["topresponsivecontainer","utilityresponsivecontainer","bodyresponsivecontainer","rightrailresponsivecontainer","footer"],":items":{"topresponsivecontainer":{"columnClassNames":{"tagbasedbreadcrumb":"aem-GridColumn aem-GridColumn--default--9","cq:responsive":"aem-GridColumn aem-GridColumn--default--9","title":"aem-GridColumn aem-GridColumn--default--9"},"cssClassName":"","paddingLeftRight":"0px","gridclassNames":"aem-Grid aem-Grid--default--9","columns":12,"items":[{"url":null,"title":null,"description":null,"lastModified":1645984160912,"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/topresponsivecontainer/tagbasedbreadcrumb","name":"tagbasedbreadcrumb"},{"url":null,"title":null,"description":null,"lastModified":1645984160923,"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/topresponsivecontainer/title","name":"title"}],"halfGatedContent":false,"allowedComponents":{"applicable":false,"components":[]},":type":"cms-commons/components/content/container",":itemsOrder":["tagbasedbreadcrumb","title"],":items":{"tagbasedbreadcrumb":{"items":[{"url":"/CN/en","title":"Home","titleEng":"Home","active":false,"nonClickable":false},{"url":"/CN/en/applications/analytical-chemistry/liquid-chromatography/large-molecule-hplc","title":"Large Molecule HPLC","titleEng":"Large Molecule HPLC","active":false,"nonClickable":false},{"url":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200","title":"HPLC Analysis of PEG on Two Zenix^® SEC-300 Columns in Tandem","titleEng":"HPLC Analysis of PEG on Two Zenix^® SEC-300 Columns in Tandem","active":true,"nonClickable":true}],":type":"cms-commons/components/content/tagbasedbreadcrumb"},"title":{"anchorId":null,"text":"HPLC Analysis of PEG on Two Zenix^® SEC-300 Columns in Tandem","openNewTab":false,"linkURL":null,"type":"h1",":type":"cms-commons/components/content/title"}}},"utilityresponsivecontainer":{"columnClassNames":{"cq:responsive":"aem-GridColumn aem-GridColumn--default--3"},"cssClassName":"cms-container--top","paddingLeftRight":"0px","gridclassNames":"aem-Grid aem-Grid--default--3","columns":12,"items":[],"halfGatedContent":false,"allowedComponents":{"applicable":false,"components":[]},":type":"cms-commons/components/content/container",":itemsOrder":[],":items":{}},"bodyresponsivecontainer":{"columnClassNames":{"reference":"aem-GridColumn aem-GridColumn--default--9","bottomcontainer":"aem-GridColumn aem-GridColumn--default--9","dynamicproducttable":"aem-GridColumn aem-GridColumn--default--9","middleresponsivecontainer":"aem-GridColumn aem-GridColumn--default--9","topcontainer":"aem-GridColumn aem-GridColumn--default--9","cq:responsive":"aem-GridColumn aem-GridColumn--default--9"},"cssClassName":"","paddingLeftRight":"0px","gridclassNames":"aem-Grid aem-Grid--default--9","columns":12,"items":[{"url":null,"title":null,"description":null,"lastModified":1645984161037,"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/bodyresponsivecontainer/topcontainer","name":"topcontainer"},{"url":null,"title":null,"description":null,"lastModified":1645984161123,"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/bodyresponsivecontainer/dynamicproducttable","name":"dynamicproducttable"},{"url":null,"title":null,"description":null,"lastModified":1645984161133,"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/bodyresponsivecontainer/middleresponsivecontainer","name":"middleresponsivecontainer"},{"url":null,"title":null,"description":null,"lastModified":1645984161141,"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/bodyresponsivecontainer/reference","name":"reference"},{"url":null,"title":null,"description":null,"lastModified":1645984161150,"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/bodyresponsivecontainer/bottomcontainer","name":"bottomcontainer"}],"halfGatedContent":false,"allowedComponents":{"applicable":false,"components":[]},":type":"cms-commons/components/content/container",":itemsOrder":["topcontainer","dynamicproducttable","middleresponsivecontainer","reference","bottomcontainer"],":items":{"topcontainer":{"columnClassNames":{"table_97868578":"aem-GridColumn aem-GridColumn--default--12","table":"aem-GridColumn aem-GridColumn--default--12"},"cssClassName":"","paddingLeftRight":"0px","gridclassNames":"aem-Grid aem-Grid--12 aem-Grid--default--12","columns":12,"items":[{"url":null,"title":null,"description":null,"lastModified":1645984161071,"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/bodyresponsivecontainer/topcontainer/table","name":"table"},{"url":null,"title":null,"description":null,"lastModified":1645984161109,"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/bodyresponsivecontainer/topcontainer/table_97868578","name":"table_97868578"}],"halfGatedContent":false,"allowedComponents":{"applicable":false,"components":[]},":type":"cms-commons/components/content/container",":itemsOrder":["table","table_97868578"],":items":{"table":{"anchorId":null,"enableSort":false,"verticalTable":false,"headline":"Conditions","title":"Table 1","caption":null,"table":"\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n

Column	Zenix SEC-300 (2 ea), 30 cm x 7.8 mm I.D., 3 µm partcles, 300 Ã…, connected in tandem (Z777033)
Column Temp.	25 °C
Mobile Phase	150 mM Sodium Phosphate Buffer pH=7.0
Flow Rate	0.5 mL/min
Sample	1.PEG 81,400 Da (2 mg/mL) \r\n2.PEG 50,630 Da (2 mg/mL) \r\n3.PEG 40,000 Da (2 mg/mL) \r\n4.PEG 26,100 Da (2 mg/mL) \r\n5.PEG 8,730 Da (2 mg/mL)
Injection	10 µL
Detector	Refractive Index at 30 °C

\r\n",":type":"cms-commons/components/content/table"},"table_97868578":{"anchorId":null,"enableSort":false,"verticalTable":false,"headline":"Description","title":"Table 2","caption":null,"table":"\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n

Categories	Analytical Chromatography, Proteins
Featured Industry	Life Science and Biopharma
General Description	Pegylation of biotherapeutic drugs improves solubility, increases half life in the blood stream and reduces immunogenicity. Size exclusion chromatography (SEC) is often applied to characterize protein–polyethylene glycol (PEG) conjugates and establish the number of attached PEG chains, since SEC is a precise and robust technique that combines ease of set up and strainghtforward method develpopment. \r\nThis chromatogram shows the separation of four polyethyleneglycol polymers varying in molecular mass 8730 to 81,400 Da on two Zenix^® SEC-300 Columns in series.
Legal Information	Zenix is a registered trademark of Sepax Technologies
Suitability	Application for HPLC

\r\n",":type":"cms-commons/components/content/table"}}},"dynamicproducttable":{"anchorId":null,"productsArray":["5.33001 Supelco (MM)","1.00029 Supelco (MM)","73594 Supelco (SIAL)","70221 Supelco (SIAL)","1.11727 Supelco (MM)","5.33002 Supelco (MM)","1.06007 Supelco (MM)","1.06035 Supelco (MM)","1.06035 Supelco (MM)","342483 Sigma-Aldrich (ALDRICH)","69372 Millipore (SIAL)","1.15333 Supelco (MM)","Z777033 Supelco (SUPELCO)"],"isShowImage":false,"maxRows":20,":type":"cms-commons/components/content/dynamicproducttable","tableTitle":"Materials","products":[{"productKey":"533001","brandKey":"MM"},{"productKey":"100029","brandKey":"MM"},{"productKey":"73594","brandKey":"SIAL"},{"productKey":"70221","brandKey":"SIAL"},{"productKey":"111727","brandKey":"MM"},{"productKey":"533002","brandKey":"MM"},{"productKey":"106007","brandKey":"MM"},{"productKey":"106035","brandKey":"MM"},{"productKey":"106035","brandKey":"MM"},{"productKey":"342483","brandKey":"ALDRICH"},{"productKey":"69372","brandKey":"SIAL"},{"productKey":"115333","brandKey":"MM"},{"productKey":"Z777033","brandKey":"SUPELCO"}]},"middleresponsivecontainer":{"columnClassNames":{},"cssClassName":"","paddingLeftRight":"0px","gridclassNames":"aem-Grid aem-Grid--12 aem-Grid--default--12","columns":12,"items":[],"halfGatedContent":false,"allowedComponents":{"applicable":false,"components":[]},":type":"cms-commons/components/content/container",":itemsOrder":[],":items":{}},"reference":{"headline":null,"anchorId":null,"startNumber":null,"references":null,":type":"cms-commons/components/content/reference"},"bottomcontainer":{"columnClassNames":{},"cssClassName":"","paddingLeftRight":"0px","gridclassNames":"aem-Grid aem-Grid--12 aem-Grid--default--12","columns":12,"items":[],"halfGatedContent":false,"allowedComponents":{"applicable":false,"components":[]},":type":"cms-commons/components/content/container",":itemsOrder":[],":items":{}}}},"rightrailresponsivecontainer":{"columnClassNames":{"relatedcontentlinks_":"aem-GridColumn aem-GridColumn--default--3","cq:responsive":"aem-GridColumn aem-GridColumn--default--3","relatedcontentlinks__633835601":"aem-GridColumn aem-GridColumn--default--3"},"cssClassName":"","paddingLeftRight":"0px","gridclassNames":"aem-Grid aem-Grid--default--3","columns":12,"items":[{"url":null,"title":null,"description":null,"lastModified":1606315066789,"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/rightrailresponsivecontainer/relatedcontentlinks_","name":"relatedcontentlinks_"},{"url":null,"title":null,"description":null,"lastModified":1606315124502,"path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200/jcr:content/root/rightrailresponsivecontainer/relatedcontentlinks__633835601","name":"relatedcontentlinks__633835601"}],"halfGatedContent":false,"allowedComponents":{"applicable":false,"components":[]},":type":"cms-commons/components/content/container",":itemsOrder":["relatedcontentlinks_","relatedcontentlinks__633835601"],":items":{"relatedcontentlinks_":{"contentType":"sigma-aldrich:content-type/page-type/protocol","linksNumber":8,"anchorId":null,"rightRail":false,"ctaLink":"/CN/en/search/facet-search?focus=sitecontent&term=facet-search&facet=facet_content_type%3AProtocol&facet=facet_applications%3ALarge+molecule+HPLC","title":"Related Articles","tags":["sigma-aldrich:applications/analytical-chemistry/large-molecule-hplc"],"ctaTitle":null,"categories":[{"categoryTitle":"Syringe Care","categoryUrl":"/CN/en/technical-documents/protocol/analytical-chemistry/gas-chromatography/general-syringe-care","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image/default-link-image.png","externalUrl":false},{"categoryTitle":"Analysis of a Bispecific Monoclonal Antibody Using Size Exclusion Chromatography and Mass Spectrometry","categoryUrl":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/analysis-bispecific-monoclonal-antibody","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image/default-link-image.png","externalUrl":false},{"categoryTitle":"Empowering biochemists to analyze glycoproteins with exceptional reproducibility","categoryUrl":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/bioshell-glycan-hplc-columns","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image/default-link-image.png","externalUrl":false},{"categoryTitle":"BIOshell™ IgG 1000 Å C4 UHPLC Column for Improved Biomacromolecule Separations","categoryUrl":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/bioshell-uhplc-column-for-improved-biomacromolecule-separations","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image/default-link-image.png","externalUrl":false},{"categoryTitle":"Calibration Curves for TSKgel® SW mAb Columns","categoryUrl":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/calibration-curves-for-tskgel-sw-mab-columns-g006277","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image/default-link-image.png","externalUrl":false},{"categoryTitle":"A Fast and Reliable Method for Purity Analysis of Filgrastim","categoryUrl":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/filgrastim-purity-analysis","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image/default-link-image.png","externalUrl":false},{"categoryTitle":"HPLC Analysis of a Procainamide-Labeled Dextran Ladder on BIOshell™ Glycan using HILIC-FLR","categoryUrl":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-a-procainamide-labeled-dextran-ladder-g006408","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image/default-link-image.png","externalUrl":false},{"categoryTitle":"HPLC Analysis of Bovine Serum Albumin (BSA) on Zenix® SEC-300 versus SRT® SEC-300, Effect of Particle Size on Resolution","categoryUrl":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image/default-link-image.png","externalUrl":false}],":type":"cms-commons/components/content/relatedcontentlinks"},"relatedcontentlinks__633835601":{"linksNumber":8,"anchorId":null,"rightRail":false,"ctaLink":"","title":"Related Product Categories","tags":null,"ctaTitle":null,"categories":[{"categoryTitle":"HPLC Columns","categoryUrl":"/CN/en/products/analytical-chemistry/analytical-chromatography/hplc-columns","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image/default-link-image.png","externalUrl":false},{"categoryTitle":"Salts","categoryUrl":"/CN/en/products/chemistry-and-biochemicals/lab-chemicals/salts","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image/default-link-image.png","externalUrl":false}],":type":"cms-commons/components/content/relatedcontentlinks"}}},"footer":{"richText":false,":type":"cms-commons/components/content/footer"}},":type":"wcm/foundation/components/responsivegrid"}},":hierarchyType":"page",":path":"/CN/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-peg-g006200"}