{"metaDataObj":{"isNoIndex":false,"isNoFollow":false,"keywords":["Filtration","Thin layer chromatography","Protocol","Calibration, qualification, and validation"],"title":"TLC Analysis of Caffeine in Coffee Samples, Quantification of Analyte Concentration","seoSchemas":[{"@context":"https://schema.org","@type":"BreadcrumbList","itemListElement":[{"@type":"ListItem","position":1,"name":"Thin Layer Chromatography","item":"%%HOMEPAGE_URL%%/CN/en/applications/analytical-chemistry/thin-layer-chromatography"},{"@type":"ListItem","position":2,"name":"TLC Analysis of Caffeine in Coffee Samples, Quantification of Analyte Concentration"}]}],"description":"Separation of Caffiene","pageType":"Protocol"},"title":"TLC Analysis of Caffeine in Coffee Samples, Quantification of Analyte Concentration","designPath":"/libs/settings/wcm/designs/default","lastModifiedDate":1629540401289,"templateName":"technical-article---protocol-page-template","cssClassNames":"page basicpage","language":"en",":type":"cms-commons/components/structure/page",":items":{"root":{"columnCount":12,"columnClassNames":{"rightrailresponsivecontainer":"aem-GridColumn aem-GridColumn--default--3 aem-GridColumn--offset--default--0 aem-GridColumn--default--none","footer":"aem-GridColumn aem-GridColumn--default--12","utilityresponsivecontainer":"aem-GridColumn aem-GridColumn--default--3 aem-GridColumn--offset--default--0 aem-GridColumn--default--none","bodyresponsivecontainer":"aem-GridColumn aem-GridColumn--default--9 aem-GridColumn--offset--default--0 aem-GridColumn--default--none","topresponsivecontainer":"aem-GridColumn aem-GridColumn--default--9 aem-GridColumn--offset--default--0 aem-GridColumn--default--none"},"gridClassNames":"aem-Grid aem-Grid--12 aem-Grid--default--12","allowedComponents":{"applicable":false,"components":[{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/aboutus","title":"About Us Text"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/accordion","title":"Accordion"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/audio","title":"Audio"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/author","title":"Author"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/breadcrumb","title":"Breadcrumbs - Page Path"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/button","title":"Button"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/card","title":"Card"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/carousel","title":"Carousel"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/categorysearchresults","title":"Category Search Results"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/cftext","title":"CF Text"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/contact","title":"Contact Component"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/container","title":"Container component"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/dealerlicense","title":"Dealer License"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/dynamicproducttable","title":"Dynamic Product Table"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/event-sidebar","title":"Event Sidebar"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/eventlisting","title":"Event Listing"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/experiencefragment","title":"Experience Fragment"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/featuretiles","title":"Feature Tiles"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/footer","title":"Footer"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/formAssembly","title":"Form Assembly"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/gatedbutton","title":"Gated Button"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/gatedcontent","title":"Gated Content"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/globalfooter","title":"Global Footer"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/header","title":"Header"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/herocomponent","title":"Hero Component"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/workflows/horizontal","title":"Workflow Horizontal"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/horizontaltab","title":"Tabbed Horizontal Navigation"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/image","title":"Image"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/leftnavigation","title":"Navigation Model"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/locationmap","title":"Map"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/pressreleasegrid","title":"Related Press releases"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/pressreleaselisting","title":"Press release listing"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/presstext","title":"Region Text"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/primarycontent","title":"Primary Content"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/printsocialsharing","title":"Print Social sharing"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/promobanner","title":"Promo Banner"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/rawhtml","title":"Raw HTML"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/recommendation","title":"Recommendation"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/reference","title":"Reference"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/relatedContentCarousel","title":"Related Content Carousel"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/relatedcontentgrid","title":"Related Content Grid"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/relatedcontentlinks","title":"Related Content Links"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/relatedproductresources","title":"Related Product Resources"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/relatedproducts","title":"Related Products Carousel"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/relatedproductsrightrail","title":"Related Products Right Rail"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/relatedwebinar","title":"Related Webinar"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/sectiondivider","title":"Section Divider"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/sitecontentsearch","title":"Site Content Search"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/speakerpage","title":"Speaker Page"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/speakers-list","title":"Speakers List"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/table","title":"Static Table"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/tagbasedbreadcrumb","title":"Breadcrumbs - Tag Based"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/text","title":"Text"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/title","title":"Title"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/topnavigation","title":"Top Navigation"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/upcomingeventsgrid","title":"Upcoming Events"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/workflows/vertical","title":"Workflow Vertical"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/video","title":"Video"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/webinarlisting","title":"Webinar listing"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/webinarsidebar","title":"Webinar Sidebar"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/cms-commons/components/content/worldwideofficelisting","title":"Worldwide Office Listing"}]},":items":{"topresponsivecontainer":{"columnClassNames":{"tagbasedbreadcrumb":"aem-GridColumn aem-GridColumn--default--9","cq:responsive":"aem-GridColumn aem-GridColumn--default--9","title":"aem-GridColumn aem-GridColumn--default--9"},"cssClassName":"","paddingLeftRight":"0px","gridclassNames":"aem-Grid aem-Grid--default--9","columns":12,"items":[{"url":null,"title":null,"description":null,"lastModified":1629540401077,"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/topresponsivecontainer/tagbasedbreadcrumb","name":"tagbasedbreadcrumb"},{"url":null,"title":null,"description":null,"lastModified":1629540401084,"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/topresponsivecontainer/title","name":"title"}],"halfGatedContent":false,"allowedComponents":{"applicable":false,"components":[]},":type":"cms-commons/components/content/container",":items":{"tagbasedbreadcrumb":{"items":[{"url":"/CN/en","title":"Home","titleEng":"Home","active":false,"nonClickable":false},{"url":"/CN/en/applications/analytical-chemistry/thin-layer-chromatography","title":"Thin Layer Chromatography","titleEng":"Thin Layer Chromatography","active":false,"nonClickable":false},{"url":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples","title":"TLC Analysis of Caffeine in Coffee Samples, Quantification of Analyte Concentration","titleEng":"TLC Analysis of Caffeine in Coffee Samples, Quantification of Analyte Concentration","active":true,"nonClickable":true}],":type":"cms-commons/components/content/tagbasedbreadcrumb"},"title":{"anchorId":null,"text":"TLC Analysis of Caffeine in Coffee Samples, Quantification of Analyte Concentration","openNewTab":false,"linkURL":null,"type":"h1",":type":"cms-commons/components/content/title"}},":itemsOrder":["tagbasedbreadcrumb","title"]},"utilityresponsivecontainer":{"columnClassNames":{"cq:responsive":"aem-GridColumn aem-GridColumn--default--3"},"cssClassName":"cms-container--top","paddingLeftRight":"0px","gridclassNames":"aem-Grid aem-Grid--default--3","columns":12,"items":[],"halfGatedContent":false,"allowedComponents":{"applicable":false,"components":[]},":type":"cms-commons/components/content/container",":items":{},":itemsOrder":[]},"bodyresponsivecontainer":{"columnClassNames":{"reference":"aem-GridColumn aem-GridColumn--default--9","bottomcontainer":"aem-GridColumn aem-GridColumn--default--9","dynamicproducttable":"aem-GridColumn aem-GridColumn--default--9","middleresponsivecontainer":"aem-GridColumn aem-GridColumn--default--9","topcontainer":"aem-GridColumn aem-GridColumn--default--9","cq:responsive":"aem-GridColumn aem-GridColumn--default--9"},"cssClassName":"","paddingLeftRight":"0px","gridclassNames":"aem-Grid aem-Grid--default--9","columns":12,"items":[{"url":null,"title":null,"description":null,"lastModified":1629540401153,"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/bodyresponsivecontainer/topcontainer","name":"topcontainer"},{"url":null,"title":null,"description":null,"lastModified":1629540401214,"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/bodyresponsivecontainer/dynamicproducttable","name":"dynamicproducttable"},{"url":null,"title":null,"description":null,"lastModified":1629540401222,"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/bodyresponsivecontainer/middleresponsivecontainer","name":"middleresponsivecontainer"},{"url":null,"title":null,"description":null,"lastModified":1629540401227,"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/bodyresponsivecontainer/reference","name":"reference"},{"url":null,"title":null,"description":null,"lastModified":1629540401233,"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/bodyresponsivecontainer/bottomcontainer","name":"bottomcontainer"}],"halfGatedContent":false,"allowedComponents":{"applicable":false,"components":[]},":type":"cms-commons/components/content/container",":items":{"topcontainer":{"columnClassNames":{"image":"aem-GridColumn aem-GridColumn--default--9 aem-GridColumn--offset--default--0 aem-GridColumn--default--none","table_1483721786":"aem-GridColumn aem-GridColumn--default--12","table":"aem-GridColumn aem-GridColumn--default--12"},"cssClassName":"","paddingLeftRight":"0px","gridclassNames":"aem-Grid aem-Grid--12 aem-Grid--default--12","columns":12,"items":[{"url":null,"title":null,"description":null,"lastModified":1629540401177,"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/bodyresponsivecontainer/topcontainer/image","name":"image"},{"url":null,"title":null,"description":null,"lastModified":1629540401196,"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/bodyresponsivecontainer/topcontainer/table","name":"table"},{"url":null,"title":null,"description":null,"lastModified":1629540401204,"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/bodyresponsivecontainer/topcontainer/table_1483721786","name":"table_1483721786"}],"halfGatedContent":false,"allowedComponents":{"applicable":false,"components":[]},":type":"cms-commons/components/content/container",":items":{"image":{"anchorId":null,"title":"TLC Analysis of Caffeine in Coffee Samples, Quantification of Analyte Concentration","link":"","src":"/deepweb/assets/sigmaaldrich/marketing/global/images/technical-documents/articles/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/tlc-analysis-of-caffeine-in-coffee-samples.jpg","alt":"TLC Analysis of Caffeine in Coffee Samples, Quantification of Analyte Concentration","externalUrl":false,"widths":[],":type":"cms-commons/components/content/image","caption":"","displayPopupTitle":true},"table":{"anchorId":null,"enableSort":false,"verticalTable":false,"headline":"Conditions","title":null,"caption":null,"table":"\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n

sample preparation	The boiled coffee samples were extracted and filtered with a 0.45 μm Millex syringe filter.
plate	HPTLC Silica gel 60 F254, 20 cm x 10 cm (1.05642.0001)
eluent	[A] acetonitrile; [B] methanol; [C] n-Heptane; (6:4:1, A:B:C)
migration distance	5 cm
sample	0.1 μL to 2.0 μL
detector	UV, 254 nm

\r\n",":type":"cms-commons/components/content/table"},"table_1483721786":{"anchorId":null,"enableSort":false,"verticalTable":false,"headline":"Description","title":null,"caption":null,"table":"\r\n\r\n\r\n

Analysis Note

\r\n

Abstract: HPTLC (High Performance Thin Layer Chromatography) is a very useful analytical technique with very low sample preparation. Shown in the example: the quantification of caffeine in different coffee samples. The boiled coffee samples
\r\nwere extracted and filtered with a 0.45 μm syringe filter. The plate was pre-conditioned with the mobile phase. After chromatographic separation the plate was scanned with a TLC-Scanner and subsequently quantified.
\r\n
\r\nResults: Caffeine was detected under UV light (254 nm, hRf = 56, Fig. 1), (quantification information Fig. 2). A triple determination of each sample is possible in parallel.
\r\n
\r\nConclusion: These results demonstrate that caffeine can be easily quantified by TLC with a three-fold determination and minimal sample preparation.

\r\n",":type":"cms-commons/components/content/table"}},":itemsOrder":["image","table","table_1483721786"]},"dynamicproducttable":{"anchorId":"materials","productsArray":["1.00030 Supelco (MM)","Z126306 Supelco (ALDRICH)","Z264385 Aldrich (ALDRICH)","Z264393 Supelco (ALDRICH)","Z264407 Supelco (ALDRICH)","Z264415 Aldrich (ALDRICH)","1.04390 Supelco (MM)","1.05642 Supelco (MM)","Z543241 Sigma-Aldrich (ALDRICH)","Z543268 Sigma-Aldrich (ALDRICH)","Z543276 Sigma-Aldrich (ALDRICH)","Z266019 Supelco (ALDRICH)","Z266000 Supelco (ALDRICH)","1.06007 Supelco (MM)","58005 Supelco (SUPELCO)","Z204226 Supelco (ALDRICH)","Z204153 Supelco (ALDRICH)","Z126195 Supelco (ALDRICH)","S1509 Supelco (SIGMA)","Z169595 Supelco (ALDRICH)","Z169595 Supelco (ALDRICH)","Z169609 Supelco (ALDRICH)","Z169625 Supelco (ALDRICH)","Z169641 Supelco (ALDRICH)"],"isShowImage":true,"maxRows":20,":type":"cms-commons/components/content/dynamicproducttable","products":[{"productKey":"100030","brandKey":"MM"},{"productKey":"Z126306","brandKey":"ALDRICH"},{"productKey":"Z264385","brandKey":"ALDRICH"},{"productKey":"Z264393","brandKey":"ALDRICH"},{"productKey":"Z264407","brandKey":"ALDRICH"},{"productKey":"Z264415","brandKey":"ALDRICH"},{"productKey":"104390","brandKey":"MM"},{"productKey":"105642","brandKey":"MM"},{"productKey":"Z543241","brandKey":"ALDRICH"},{"productKey":"Z543268","brandKey":"ALDRICH"},{"productKey":"Z543276","brandKey":"ALDRICH"},{"productKey":"Z266019","brandKey":"ALDRICH"},{"productKey":"Z266000","brandKey":"ALDRICH"},{"productKey":"106007","brandKey":"MM"},{"productKey":"58005","brandKey":"SUPELCO"},{"productKey":"Z204226","brandKey":"ALDRICH"},{"productKey":"Z204153","brandKey":"ALDRICH"},{"productKey":"Z126195","brandKey":"ALDRICH"},{"productKey":"S1509","brandKey":"SIGMA"},{"productKey":"Z169595","brandKey":"ALDRICH"},{"productKey":"Z169595","brandKey":"ALDRICH"},{"productKey":"Z169609","brandKey":"ALDRICH"},{"productKey":"Z169625","brandKey":"ALDRICH"},{"productKey":"Z169641","brandKey":"ALDRICH"}],"tableTitle":"Materials"},"middleresponsivecontainer":{"columnClassNames":{},"cssClassName":"","paddingLeftRight":"0px","gridclassNames":"aem-Grid aem-Grid--12 aem-Grid--default--12","columns":12,"items":[],"halfGatedContent":false,"allowedComponents":{"applicable":false,"components":[]},":type":"cms-commons/components/content/container",":items":{},":itemsOrder":[]},"reference":{"headline":null,"anchorId":null,"startNumber":null,"references":null,":type":"cms-commons/components/content/reference"},"bottomcontainer":{"columnClassNames":{},"cssClassName":"","paddingLeftRight":"0px","gridclassNames":"aem-Grid aem-Grid--12 aem-Grid--default--12","columns":12,"items":[],"halfGatedContent":false,"allowedComponents":{"applicable":false,"components":[]},":type":"cms-commons/components/content/container",":items":{},":itemsOrder":[]}},":itemsOrder":["topcontainer","dynamicproducttable","middleresponsivecontainer","reference","bottomcontainer"]},"rightrailresponsivecontainer":{"columnClassNames":{"relatedcontentlinks_":"aem-GridColumn aem-GridColumn--default--3","cq:responsive":"aem-GridColumn aem-GridColumn--default--3","relatedcontentlinks__122587570":"aem-GridColumn aem-GridColumn--default--3"},"cssClassName":"","paddingLeftRight":"0px","gridclassNames":"aem-Grid aem-Grid--default--3","columns":12,"items":[{"url":null,"title":null,"description":null,"lastModified":1606188318851,"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/rightrailresponsivecontainer/relatedcontentlinks_","name":"relatedcontentlinks_"},{"url":null,"title":null,"description":null,"lastModified":1606188361619,"path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples/jcr:content/root/rightrailresponsivecontainer/relatedcontentlinks__122587570","name":"relatedcontentlinks__122587570"}],"halfGatedContent":false,"allowedComponents":{"applicable":false,"components":[]},":type":"cms-commons/components/content/container",":items":{"relatedcontentlinks_":{"contentType":"sigma-aldrich:content-type/page-type/protocol","linksNumber":8,"anchorId":null,"rightRail":false,"ctaLink":"/CN/en/search/facet-search?focus=sitecontent&term=facet-search&facet=facet_content_type%3AProtocol&facet=facet_applications%3AThin+layer+chromatography","title":"Related Articles","tags":["sigma-aldrich:applications/analytical-chemistry/thin-layer-chromatography"],"ctaTitle":null,"categories":[{"categoryTitle":"Detection and Determination of Caffeine, Taurine and Arginine in Shampoos by HPTLC","categoryUrl":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/caffeine-taurine-and-arginine-in-shampoos-by-hptlc","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image/default-link-image.png","externalUrl":false},{"categoryTitle":"Catalyst Screening followed by TLC-MS for Reaction Progress Analysis using KitAlysis™","categoryUrl":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/catalyst-screening-followed-by-tlc-ms","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image/default-link-image.png","externalUrl":false},{"categoryTitle":"Chromatographic Testing of Black Pepper According to the United States Pharmacopeia","categoryUrl":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/chromatographic-testing-of-black-pepper-according-to-the-usp","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image/default-link-image.png","externalUrl":false},{"categoryTitle":"Determination of Caffeine in Energy Drinks with HPTLC-MS","categoryUrl":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/determination-of-caffeine-in-energy-drinks","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image/default-link-image.png","externalUrl":false},{"categoryTitle":"HPTLC Application for Passiflora incarnata","categoryUrl":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/hptlc-application-passiflora-incarnata","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image/default-link-image.png","externalUrl":false},{"categoryTitle":"PLC Plates for Separations from Milligrams to Grams","categoryUrl":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/plc-plates","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image/default-link-image.png","externalUrl":false},{"categoryTitle":"Qualitative Thin Layer Chromatography Analysis Ginkgo","categoryUrl":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/qualitative-thin-layer-chromatography-analysis-ginkgo","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image/default-link-image.png","externalUrl":false},{"categoryTitle":"Determination of Caffeine in Coffee using High Performance Thin Layer Chromatography","categoryUrl":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/quantification-of-caffeine","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image/default-link-image.png","externalUrl":false}],":type":"cms-commons/components/content/relatedcontentlinks"},"relatedcontentlinks__122587570":{"linksNumber":8,"anchorId":null,"rightRail":false,"ctaLink":"","title":"Related Product Categories","tags":null,"ctaTitle":null,"categories":[{"categoryTitle":"Thin Layer Chromatography (TLC)","categoryUrl":"/CN/en/products/analytical-chemistry/analytical-chromatography/tlc-plates-and-adsorbants","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image/default-link-image.png","externalUrl":false}],":type":"cms-commons/components/content/relatedcontentlinks"}},":itemsOrder":["relatedcontentlinks_","relatedcontentlinks__122587570"]},"footer":{"richText":false,":type":"cms-commons/components/content/footer"}},":itemsOrder":["topresponsivecontainer","utilityresponsivecontainer","bodyresponsivecontainer","rightrailresponsivecontainer","footer"],":type":"wcm/foundation/components/responsivegrid"}},":itemsOrder":["root"],":hierarchyType":"page",":path":"/CN/en/technical-documents/protocol/analytical-chemistry/thin-layer-chromatography/tlc-analysis-of-caffeine-in-coffee-samples"}