TissueFab^® bioink 

TissueFab^® bioink -(GelHAHep)MA Vis/405 nm, low endotoxin formulation can be used to bioprint cell-laden hydrogels in the desired shape without any supporting material. This bioink can be crosslinked in one step using exposure to 405 nm light for further culture and maturation of cells for tissue engineering and regenerative medicine applications.

Currently, there is a need for high-quality, commercially available ready-to-use bioink formulations to enable the reproducible fabrication of synthetic tissues and organs by 3D Bioprinting

• drug discovery
• in-vitro disease models
• regenerative medicine-cell-cultured meat

Heparin is a naturally occurring linear biopolymer and highly sulfated glycosaminoglycan (GAG). Research has demonstrated that heparin can modulate binding extracellular matrix proteins and sequester growth factors and cytokines, making them useful in 3D applications.

The methacrylate-functionalization of heparin allows thermal or photochemical crosslinking via covalent conjugation. Heparins exhibit high anionic charge densities to promote large swelling ratios in water. Heparin-based bioinks are used in tissue engineering, 3D bio-printing, and drug delivery applications.

Gelatin methacryloyl (GelMA) is a polymerizable hydrogel material derived from natural extracellular matrix (ECM) components. Due to its low cost, abundance, and retention of natural cell binding motifs, gelatin has become a highly sought material for tissue engineering applications. The addition of photocrosslinkable methacrylamide functional groups in GelMA allows the synthesis of biocompatible, biodegradable, and non-immunogenic hydrogels that are stable in biologically relevant conditions and promote cell adhesion, spreading, and proliferation.

Hyaluronic acid (HA) is a linear polysaccharide of alternating D-glucuronic acid/N-acetyl-D-glucosamine found primarily in connective tissues. HA-based hydrogels are prevalent in tissue engineering, 3D bioprinting, and drug delivery applications. The methacrylate (MA) functionalized hyaluronic acid is photo-cross-linkable.

Low Endotoxin, low bioburden: Endotoxins can negatively impact cellular growth, morphology, differentiation, inflammation, and protein expression. The bioburden is the number of contaminated organisms found in a given amount of material.

We test each lot for endotoxins in addition to total bioburden (aerobic and fungal) to minimize unwanted interactions. For more information: Cell Culture FAQs: Bacterial Endotoxin Contamination

TISSUEFAB is a registered trademark of Merck KGaA, Darmstadt, Germany