05-386
Anti-Vinculin Antibody
UPSTATE®, mouse monoclonal, V284
别名:
Anti-CMD1W, Anti-CMH15, Anti-HEL114, Anti-MV, Anti-MVCL
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选择尺寸
变更视图
关于此项目
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
V284, monoclonal
Species reactivity:
human, rat, mouse, rabbit, chicken
Application:
IHC, IP, WB
Citations:
41
产品名称
抗纽蛋白抗体，克隆V284, clone V284, Upstate®, from mouse
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
V284, monoclonal
species reactivity
human, rat, mouse, rabbit, chicken
manufacturer/tradename
Upstate®
technique(s)
immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... VCL(7414)
General description
117kDa
Immunogen
人黏着斑蛋白
Application
抗黏着斑蛋白抗体，克隆V284，可检测黏着斑蛋白的水平 & 已出版&并经过验证可用于IP、WB、IH。
研究子类别
细胞骨架信号转导
细胞骨架信号转导
研究类别
信号传导
信号传导
Biochem/physiol Actions
黏着斑蛋白
Physical form
PBS，pH 7.4，含0.05%叠氮化钠和1 mg/ml BSA
形式：纯化
蛋白A层析
Preparation Note
在-20°C下可保存2年
Analysis Note
对照
阳性抗原对照：货号12-301，未刺激的A431细胞裂解液。加入2.5µL 2-巯基乙醇/100µL裂解液，煮沸5分钟以减少制备量。在微凝胶每个泳道上样20µg经还原的裂解液。
阳性抗原对照：货号12-301，未刺激的A431细胞裂解液。加入2.5µL 2-巯基乙醇/100µL裂解液，煮沸5分钟以减少制备量。在微凝胶每个泳道上样20µg经还原的裂解液。
通过免疫印迹对人A431细胞的RIPA裂解液，鸡胚成纤维细胞（CEF）或小鼠3T3/A31成纤维细胞的裂解液进行常规评估
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Emily M Mace et al.
Blood, 116(8), 1272-1279 (2010-05-18)
The leukocyte integrin LFA-1 is critical for natural killer (NK) cell cytotoxicity as it mediates NK-cell adhesion to target cells and generates activating signals that lead to polarization of the actin cytoskeleton. However, the LFA-1-mediated signaling pathway is not fully
Sunil K Parapuram et al.
Investigative ophthalmology & visual science, 50(12), 5965-5974 (2009-07-07)
In proliferative vitreoretinopathy retinal pigment epithelial (RPE) cells undergo epithelial-mesenchymal transformation (EMT). Vitreous and transforming growth factor-beta (TGFbeta) have been implicated in this EMT. The role of TGFbeta in the vitreous-mediated transformation of low-passage human RPE cells was investigated. Cells
Diletta Dolfini et al.
Scientific reports, 9(1), 12955-12955 (2019-09-12)
NF-Y is a CCAAT-binding trimeric transcription factor, whose regulome, interactome and oncogenic potential point to direct involvement in cellular transformation. Yet little is known about the levels of NF-Y subunits in tumors. We focused on breast carcinomas, and analyzed RNA-Seq