05-531
Anti-His Tag Antibody
clone 4D11, mouse monoclonal, Upstate
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关于此项目
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.43
Clone:
4D11, monoclonal
Technique(s):
immunocytochemistry: suitable, western blot: suitable
Application:
ICC, WB
Citations:
7
产品名称
Anti-Histidine Tagged Antibody, clone 4D11, clone 4D11, Upstate®, from mouse
biological source
mouse
Quality Level
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
4D11, monoclonal
species reactivity (predicted by homology)
all
manufacturer/tradename
Upstate®
technique(s)
immunocytochemistry: suitable, western blot: suitable
isotype
IgG2aκ
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... HRG(3273)
General description
The apparent molecular weight is related to the tagged protein.
Immunogen
N-terminal His-tagged and truncated TRAP protein corresponding to amino acids 26-248 from P. falciparum expressed in E coli.
Application
This Anti-Histidine Tagged Antibody (anti His antibody) clone 4D11 is validated for use in IC, WB for the detection of Histidine Tagged.
Biochem/physiol Actions
Recognizes tag sequence HHHHHHGS on either the N- or C-terminus of recombinant proteins; GS amino acids are required for recognition of 6His tag
Physical form
Format: Purified
Protein G Purified immunoglobulin in Immunoaffinity Purified immunoglobulin in 0.014M phosphate buffer, pH 7.6, 0.175M NaCl, and 0.07% sodium azide.
Analysis Note
Control
Cells expressing the His-tag
Cells expressing the His-tag
routinely evaluated by immunoblot on recombinant N-terminal and C-terminal His-tagged proteins
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
K J Robson et al.
The EMBO journal, 14(16), 3883-3894 (1995-08-15)
Plasmodium sporozoites collected from oocysts, haemocoel and salivary glands of the mosquito show profound differences in their biological properties such as motility, ability to induce protective immune response and infectivity for vertebrate host cells. Sporozoites from salivary glands are much
Francesca Mancini et al.
The EMBO journal, 28(13), 1926-1939 (2009-06-13)
MDM4 is a key regulator of p53, whose biological activities depend on both transcriptional activity and transcription-independent mitochondrial functions. MDM4 binds to p53 and blocks its transcriptional activity; however, the main cytoplasmic localization of MDM4 might also imply a regulation
Frances R Wadelin et al.
PloS one, 8(2), e58052-e58052 (2013-03-06)
Preferentially expressed antigen in melanoma (PRAME) has been described as a cancer-testis antigen and is associated with leukaemias and solid tumours. Here we show that PRAME gene transcription in leukaemic cell lines is rapidly induced by exposure of cells to