05-633
抗-BrdU抗体，克隆BU-1
culture supernatant, clone BU-1, Upstate®
别名:
抗BrdU抗体
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
BU-1, monoclonal
Technique(s):
immunocytochemistry: suitable
Application:
ICC
Citations:
14
biological source
mouse
Quality Level
antibody form
culture supernatant
antibody product type
primary antibodies
clone
BU-1, monoclonal
species reactivity (predicted by homology)
all
manufacturer/tradename
Upstate®
technique(s)
immunocytochemistry: suitable
isotype
IgG2a
shipped in
wet ice
target post-translational modification
unmodified
General description
掺入DNA的溴脱氧尿苷（BrdU）的免疫细胞化学检测是研究正常细胞和赘生性细胞的细胞动力学的有力工具。用胸苷类似物BrdU体外或体内标记肿瘤细胞，然后用特异性抗BrdU单克隆抗体检测掺入的BrdU是定量DNA合成程度的准确而全面的方法。BrdU被掺入S期细胞的新合成DNA中，因此可以提供S期细胞比例的估计值。还可以通过双变量BrdU/DNA流式细胞术分析获得动态增殖信息（例如S期转运速率和潜在的倍增时间）。
未给出
Immunogen
5-碘尿苷：卵清蛋白结合物
Application
用已证明可在ICC中起作用的抗BrdU抗体（克隆BU-1（小鼠单克隆抗体））检测BrdU（也称为溴脱氧尿苷）。
Biochem/physiol Actions
5-溴-2-脱氧尿苷
Physical form
4瓶，每瓶含有2.5 mL冻干的小鼠培养物上清液。上清液含有支原体，其贡献促进抗体结合的酶。冻干粉末。
Analysis Note
通过免疫细胞化学在HeLa细胞上评价了代表性批次。代表性批次的1:2-1:4稀释液在用95%乙醇，5%冰醋酸固定的HeLa细胞中显示出抗BrdU的阳性免疫染色。使用DNA复制检测试剂盒（目录号17-316）检测该批次。
技术提示：该抗体可识别掺入双链DNA的5-溴-2-脱氧尿苷。不需要DNA变性。
技术提示：该抗体可识别掺入双链DNA的5-溴-2-脱氧尿苷。不需要DNA变性。
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
高风险级别生物产品-Merck
此项目有
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Testing null