biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
M110, monoclonal
species reactivity
rat, human, mouse
manufacturer/tradename
Upstate®
technique(s)
ELISA: suitable, immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG1
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... PRKCA(5578)
General description
80-82 kDa
Immunogen
Recombinant protein of human PKCγ amino acids 499-697.
Application
Anti-PKC (α Antibody, β, γ), clone M110 detects level of PKC (α & has been published & validated for use in ELISA, IP, WB & IC.
Biochem/physiol Actions
Recognizes PKC α, β, γ. Does not cross react with other PKC isoforms.
Physical form
100 μL of protein A purified mouse monoclonal IgG1 in 50% affinity storage buffer (PBS containing 1.0 mg/mL BSA and 0.05% sodium azide) and 50% glycerol.
Format: Purified
Preparation Note
Stable for 2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
Analysis Note
Routinely evaluated by immunoblot.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Amos M Sakwe et al.
The Journal of biological chemistry, 280(6), 4436-4441 (2004-12-02)
This study examined the mechanism of Ca2+ entry and the role of protein kinase C (PKC) in Ca2+ signaling induced by activation of the calcium sensing receptor (CaR) in HEK293 cells stably expressing the CaR. We demonstrate that influx of
Michael Rosenberg et al.
Molecular biology of the cell, 17(3), 1364-1374 (2006-01-06)
Nonmuscle myosin II is an important component of the cytoskeleton, playing a major role in cell motility and chemotaxis. We have previously demonstrated that, on stimulation with epidermal growth factor (EGF), nonmuscle myosin heavy chain II-B (NMHC-IIB) undergoes a transient
Lilian Kisiswa et al.
Open biology, 7(1) (2017-01-20)
Tumour necrosis factor receptor 1 (TNFR1)-activated TNFα reverse signalling, in which membrane-integrated TNFα functions as a receptor for TNFR1, enhances axon growth from developing sympathetic neurons and plays a crucial role in establishing sympathetic innervation. Here, we have investigated the
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| 货号 | GTIN |
|---|---|
| 05-983 | 04053252671845 |