biological source
rabbit
Quality Level
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable (ChIP-seq), cell based assay: suitable, dot blot: suitable, multiplexing: suitable, western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
dimethylation (Lys27)
Gene Information
human ... H3C1(8350)
General description
17 kDa
抗-二甲基-组蛋白H3(Lys27)
Immunogen
KLH缀合合成2X支肽,包含序列...ARme2KSA...,其中me2K对应于人组蛋白H3残基27处的二甲基赖氨酸。
Application
其他参考的研究应用:
斑点印迹,免疫细胞化学(细胞)
斑点印迹,免疫细胞化学(细胞)
使用在Mplex、PIA、WB、ChIP-seq、DB中验证的抗二甲基组蛋白H3(Lys27)抗体(兔多克隆抗体)检测二甲基组蛋白H3(Lys27),也称为H3K27me2,组蛋白H3(二甲基K27)。
研究子类别
组蛋白
组蛋白
研究类别
表观遗传学&核功能
表观遗传学&核功能
Biochem/physiol Actions
基于序列同源性,预期具有广泛的物种交叉反应
识别二甲基化的组蛋白H3(Lys27)。
Physical form
亲和纯化的IgG溶于0.1 M Tris-甘氨酸(pH 7.4),150 mM NaCl和0.05%NaN3中。
肽亲和纯化
Preparation Note
自运送之日起在2-8°C可稳定保存2年。
Analysis Note
对照
HeLa全细胞裂解液,U2OS细胞裂解液
HeLa全细胞裂解液,U2OS细胞裂解液
通过免疫印迹对HeLa细胞的酸提取蛋白进行了常规评估。
Other Notes
浓度:请参考批次特异性浓缩物的分析证书。
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
The Nucleolus in the Mouse Oocyte is Required for the Early Step of Both Female and Male Pronucleus Organization.
Ogushi S, Saitou M
Journal of Reproduction and Development null
Establishment of histone h3 methylation on the inactive X chromosome requires transient recruitment of Eed-Enx1 polycomb group complexes.
Silva, Jose, et al.
Developmental Cell, 4, 481-495 (2003)
Molly Gale et al.
Oncotarget, 7(26), 39931-39944 (2016-10-26)
Lysine demethylase 5A (KDM5A/RBP2/JARID1A) is a histone lysine demethylase that is overexpressed in several human cancers including lung, gastric, breast and liver cancers. It plays key roles in important cancer processes including tumorigenesis, metastasis, and drug tolerance, making it a
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