Anti-HuR Antibody

HuR (Hu-antigen R, ELAV-like protein 1, ELAV1) is an RNA binding protein that contains 3 RNA-binding domains. It is known to binds AU-rich elements if mRNA. It regulates gene expression by destabilizing their mRNA. It is known to be upregulated in certain cancers such as breast and gastric cancers. Some of its targets include cFos, IL-3, and BRCA1 mRNAs. HuR has also been shown to be involved in the regulation of VEFG-A and NFkB expression in certain cell types. It activation and accumulation are also linked to many energy signaling molcules such as that of the PI3K/Akt pathway and AMPK..

~36 kDa

KLH-conjugated, synthetic peptide corresponding to amino acids 1-13 of human HuR.

Immunoprecipitation Analysis:
10 µg from a representative lot detected HuR in RIPA lysate.

Immunocytochemistry Analysis:
A 1:500 dilution from a representative lot detected HuR in HeLa and NIH/3T3 cell lysates.

Immunohistochemistry Analysis:
This antibody has been reported by an independent laboratory to detect HuR in rabbit placental tissue.

Anti-HuR Antibody is a Rabbit Polyclonal Antibody for detection of HuR also known as ELAV-like protein 1, Hu-antigen R & has been validated in IP, WB, ICC.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

RNA Binding Protein (RBP)

HuR

Format: Purified

See datasheet for exact formulation.

See datasheet for specific purification method.

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Western Blot Analysis: Evaluated by Western Blot in 3T3/A31 cell lysate using 1 µg/mL of antibody and 10 µg of lysate.

Control
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

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