Quality Level
biological source
rabbit
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse
manufacturer/tradename
Upstate®
technique(s)
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... NR5A1(2516), SF1(7536)
General description
53kda
甾体生成因子-1(SF-1)调节参与肾上腺和性腺发育的多个基因,并参与多种激素的生物合成,包括肾上腺和性腺类固醇、抗苗勒管激素(AMH)和促性腺激素。SF-1属于孤儿核受体Fushi Tarazu因子-1(FTZ-F1)亚家族。在成人卵巢中,SF-1定位于膜/间质细胞。
Immunogen
小鼠SF-1的DNA结合域,在大肠杆菌中作为GST融合蛋白产生,通过GST琼脂糖柱层析部分纯化
Application
抗-SF-1抗体是一种高质量的兔多克隆抗体,用于检测SF-1,&已在WB中得到验证。
研究子类别
转录因子
转录因子
研究类别
表观遗传学&核功能
表观遗传学&核功能
Biochem/physiol Actions
识别SF-1。
Physical form
形式:纯化
纯化蛋白A
蛋白A纯化的免疫球蛋白,溶于30%甘油,0.07M Tris-甘氨酸(pH 7.4)、0.105 M NaCl,0.035%叠氮化钠作为防腐剂的溶液中。
Preparation Note
自收到之日起在-20°C可稳定保存2年。避免反复冻融。为了最大程度地回收产品,在融化后和取下盖子之前,将原始小瓶进行离心。
Analysis Note
对照
Y1细胞裂解液
Y1细胞裂解液
通过免疫印迹对Y1细胞的RIPA裂解液进行了常规评估
Other Notes
浓度:请参考批次特异性浓缩物的检验报告。
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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存储类别
10 - Combustible liquids
wgk
WGK 2
Hormonal regulation of microRNA expression in steroid producing cells of the ovary, testis and adrenal gland.
Hu, Z; Shen, WJ; Cortez, Y; Tang, X; Liu, LF; Kraemer, FB; Azhar, S
Testing null
Steroidogenic factor 1: an essential mediator of endocrine development
Parker, Keith L, et al
Recent Progress in Hormone Research, 57, 19-36 (2002)
Bruno Ferraz-de-Souza et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 25(4), 1166-1175 (2010-12-18)
The nuclear receptor steroidogenic factor-1 (SF-1, NR5A1) is a key regulator of adrenal and gonadal biology. Disruption of SF-1 can lead to disorders of adrenal development, while increased SF-1 dosage has been associated with adrenocortical tumorigenesis. We aimed to identify