biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
rat, mouse
manufacturer/tradename
Upstate®
technique(s)
immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... CCNE1(898)
General description
Mr 52kDa
细胞周期蛋白 E 是 Cdk2 的调节亚基,在哺乳动物细胞周期中控制 G1/S 的转变。细胞周期蛋白 E 的多种同工型仅在肿瘤中表达,而在正常组织中不表达,这表明细胞周期蛋白 E 受转录后调控。体外分析表明,细胞周期蛋白 E 的这些截短的变体能够磷酸化组蛋白 H1。细胞周期蛋白 E 异常被认为是各种癌症预后较差的指标。
Immunogen
KLH偶联合成肽,对应于大鼠细胞周期蛋白E的氨基酸378-396(GVLTPPHSSKKQSSEQETE)。
Application
使用经验证可用于IP、WB & IHC的抗细胞周期蛋白E抗体(兔多克隆抗体)检测细胞周期蛋白E。
研究子类别
细胞周期,DNA 复制&修复
细胞周期,DNA 复制&修复
研究类别
表观遗传学&核功能
表观遗传学&核功能
Biochem/physiol Actions
细胞周期蛋白E1
Physical form
含有0.1%叠氮化钠和0.2%明胶的PBS 。
免疫亲和纯化
Preparation Note
自发货之日起在4°C可稳定保存1年。
Analysis Note
对照
阳性抗原对照:货号12-305,3T3/A31裂解液。加入2.5 μL 2-巯基乙醇/100 μL裂解液,煮沸5分钟以还原样本。在微凝胶每个泳道上样20 μg经还原的裂解液。
阳性抗原对照:货号12-305,3T3/A31裂解液。加入2.5 μL 2-巯基乙醇/100 μL裂解液,煮沸5分钟以还原样本。在微凝胶每个泳道上样20 μg经还原的裂解液。
已通过免疫印迹法对3T3/A31细胞的RIPA裂解液进行了常规评估
Other Notes
替代:04-223
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Y Geng et al.
Cell, 97(6), 767-777 (1999-06-25)
D-type cyclins and cyclin E represent two very distinct classes of mammalian G1 cyclins. We have generated a mouse strain in which the coding sequences of the cyclin D1 gene (Ccnd1) have been deleted and replaced by those of human
Cyclin A: function and expression during cell proliferation.
Desdouets, C, et al.
Progress in Cell Cycle Research, 1, 115-123 (1995)
The cell cycle and DNA mismatch repair.
Allen G Schroering,Michael A Edelbrock,Timothy J Richards,Kandace J Williams
Experimental Cell Research null