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1.12018

Albumin fraction V

(from bovine serum) for biochemistry

别名:

Albumin fraction V, Bovine serum albumin, Serum albumin

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化学文摘社编号:
90604-29-8
MDL number:
MFCD00000745
NACRES:
NA.21
EC Index Number:
292-322-5
UNSPSC Code:
12352202
Biological source:
bovine
Form:
solid
Technique(s):
immunoassay: suitable, western blot: suitable
Concentration:
≥97% protein (spectralphotometric; calc. on anhydrous substance), ≥98% (Assay of Albumin (Agarose gel electrophoresis))
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biological source

bovine

form

solid

concentration

≥97% protein (spectralphotometric; calc. on anhydrous substance), ≥98% (Assay of Albumin (Agarose gel electrophoresis))

technique(s)

immunoassay: suitable, western blot: suitable

pH

6.8-7.2 (20 °C, 1 g/L in H2O)

solubility

1 g/L

bulk density

150 kg/m3

storage temp.

2-8°C

General description

Bovine serum albumin (BSA) belongs to the serum albumin family with three domains and two sub-domains each. This globular, α-helical, non-glycosylated protein has 17-disulfide bonds.

Application

Albumin fraction V has been used:
  • as a component of perfusion buffer for perfusion protocol for rodent organ perfusion
  • as a blocking agent in western blot analysis
  • as a nitrated protein standard to study the effects of antibodies against 3-nitrotyrosine and to characterize the binding and detection of nitrated proteins in one-sided and sandwich immunoassays

Biochem/physiol Actions

Bovine Serum Albumin (BSA) is the chief component of cell culture media and also works as a blocking agent in enzyme-linked immunosorbent assay (ELISA). It is beneficial for embryonic stem cells (hESC) differentiation and assists the transport of drugs, hormones, and fatty acids. 

Analysis Note

Appearance (colour): slightly yellow
Appearance (description): leafs
Assay of protein (spectralphotometric; calc. on anhydrous substance): ≥ 97.0 %
Assay of Albumin (Agarose gel electrophoresis): ≥ 98.0 %
pH-value (1 %; sodium chloride solution c = 0.5 mol/ l): 6.8 - 7.2
K (Potassium): ≤ 0.02 %
Na (Sodium): ≤ 0.5 %
Mg (Magnesium): ≤ 0.01 %
Ca (Calcium): ≤ 0.05 %
Heavy metals (as lead): ≤ 0.001 %
Fats: ≤ 0.2 %
Decomposition products: not detectable
Water (according to Karl Fischer): ≤ 5.00 %
Ash (600 °C): ≤ 1 %
NADH Oxidase (NADH; pH 7.5; 25° C): not detectable
LDH (pyruvate; pH 7.5; 25° C): not detectable

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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