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113200

Anti-α-Actin Mouse mAb (1A4)

Name: Anti-α-Actin Mouse mAb (1A4)
Brand: MM

liquid, clone 1A4, Calbiochem^®

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关于此项目

NACRES:

NA.41

UNSPSC Code:

12352203

Clone:

1A4, monoclonal

Species reactivity:

baboon, rabbit, human

Application:

—

Citations:

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属性

biological source

mouse

Quality Level

100

antibody product type

primary antibodies

clone

1A4, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

baboon, rabbit, human

manufacturer/tradename

Calbiochem^®

storage condition

do not freeze

dilution

(Frozen Sections (1:50-1:75)
Immunoblotting
Paraffin Sections (1:50-1:75))

isotype

IgG2a

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... ACTA1(58)

说明

General description

Mouse monoclonal antibody. Recognizes α-actin in smooth muscle cells.

Recognizes α-actin in smooth muscle cells of blood vessels and parenchymal tissue of the intestine, testis, and ovary. Does not recognize actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric), or myocardium (α-myocardial).

Antibody Target Gene Symbol: ACTA2
Target Synonym: 0610041G09RIK, a-SMA, AAT6, ACTSA, Actvs, ALPHA ACTIN VSM, ALPHA SMA, ALPHA SMOOTH MUSCLE ACTIN, ALPHA-ACTIN 2, FLJ45296, MGC127800, RGD: 621676, Similar to vascular alpha actin, Sm alpha actin, SMalphaA, SMOOTH MUSCLE ALPHA ACTIN
Entrez Gene Name: actin, alpha 2, smooth muscle, aorta
Hu Entrez ID: 59 (Related Antibodies: 113200 )
Rat Entrez ID: 81633

This Anti-α-Actin Mouse mAb (1A4) is validated for use in Frozen Sections, Immunoblotting, Paraffin Sections for the detection of α-Actin.

Immunogen

Human Smooth Muscle

a synthetic peptide corresponding to amino acids 1-10 of human smooth muscle α-actin.

Application

Frozen Sections (1:50-1:75)

Immunoblotting (see comments)

Paraffin Sections (1:50-1:75)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 150 mM NaCl, 20 mM Tris-borate, BHC-6 carrier polysaccharides, pH 7.5.

Analysis Note

Negative Control
Fibroblasts, striated muscle, or myocardium

Positive Control
Smooth muscle cells of blood vessels and parenchymal tissue of intestine, testis, or ovary

Other Notes

Does not recognize actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric), or myocardium (α-myocardial).Prolonged fixation in buffered formalin destroys the epitope. The antibody is specific for the α-smooth muscle isoform of actin. This antibody is also reported to work for immunoblotting. Variables associated with assay conditions will dictate the proper working dilution.

Rannov-Jennsen, L., et al. 1990. Lab Invest.63, 532.
Skalli, O., et al. 1989. J. Histochem. Cytochem37, 315.
Skalli, O., et al. 1986. J. Cell. Biol. 103, 2787.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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存储类别

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c