description
Merck USA index - 14, 8468
assay
≥95% (cellulose acetate electrophoresis)
form
powder
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
impurities
≤0.02% fatty acids, ≤3% ash, ≤5% moisture
color
white to light yellow, light yellow to light brown
shipped in
ambient
storage temp.
2-8°C
General description
牛血清白蛋白(BSA、组分V、不含脂肪酸)适用于免疫诊断分析。它设计用于血清学检测、放射免疫测定和激素反应研究。它也适用于结合和运输研究。BSA采用冷乙醇提取法(Cohn法)制备。pH值:6.8-7.4。
牛血清白蛋白(BSA)是一种非糖基化球状α-螺旋蛋白,属于血清白蛋白家族。它由三个结构域组成,每个结构域有两个子结构域,并且具有17个二硫键。
Application
牛血清白蛋白(组分V、不含脂肪酸)已用作:
- 一种组分以用于各种细胞免疫电子显微镜(IEM)的阻断缓冲液
- 线粒体隔离缓冲液(MIB)和线粒体测定液(MAS),以捕获脂肪组织均质化过程中释放的游离脂肪酸
- 一种阻断缓冲液,以对食管上皮(EE)进行免疫荧光全旦染色
Biochem/physiol Actions
牛血清白蛋白(BSA)是细胞培养基中最重要的成分,有助于胚胎干细胞(hESC)的分化。它有助于药物、激素和脂肪酸的运输。BSA也被用作酶联免疫吸附试验(ELISA)的阻断剂。
Preparation Note
可能需要长达24小时才能完全溶解。
在复溶后存放于冰箱(4°C)。可能添加0.1% NaN₃作为防腐剂。储备溶液在4°C下可稳定保存3个月。
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
毒性:标准处理(A)
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
监管及禁止进口产品
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| 126575-100GM | 04055977205923 |
| 126575-10GM | 07790788048044 |