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Merck
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126593

白蛋白、牛血清、组分V、RIA和ELISA级

别名:

白蛋白、牛血清、组分V、RIA和ELISA级

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关于此项目

化学文摘社编号:
MDL number:
NACRES:
NA.25
UNSPSC Code:
12352202
Assay:
≥98% (agarose gel electrophoresis)
Form:
solid
Impurities:
≤3.0 ash, ≤5.0% moisture
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description

Merck USA index - 14, 8468

assay

≥98% (agarose gel electrophoresis)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

impurities

≤3.0 ash, ≤5.0% moisture

color

off-white

solubility

0.15 M sodium chloride: 10 mg/mL

cation traces

heavy metals: ≤10 ppm

shipped in

ambient

storage temp.

2-8°C

General description

牛血清白蛋白(BSA)是一种α-螺旋状的非糖基化球状蛋白,含有17个二硫键。
牛血清白蛋白,组分V(RIA和ELISA级)是可用作稳定剂和载体蛋白的纯化血浆蛋白。在非变性条件下通过改良Cohn方法和辛酸热激处理制备。

Application

牛血清白蛋白,组分V(RIA和ELISA级)已用于:
  • 在免疫组化(IHC)分析中用于胎盘组织切片的封闭
  • 作为Krebs缓冲液组分,用于体外葡萄糖刺激胰岛素分泌(GSIS)实验和药物/棕榈酸制备
  • 作为溶剂对照孵育小鼠全血,用于研究体内血小板聚集

Biochem/physiol Actions

牛血清白蛋白(BSA)是细胞培养基的重要成分,可运输脂肪酸、激素和药物。它是酶联免疫吸附试验(ELISA)中常用的封闭剂。

Preparation Note

在复溶后,分装并冷冻保存(-20°C)。储备溶液在-20°C下可稳定保存至多6个月。

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

毒性:标准处理(A)


存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

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Yuhong Xiao et al.
Journal of immunological methods, 384(1-2), 148-151 (2012-06-27)
The enzyme-linked immunosorbent assay (ELISA) is an extremely common and powerful laboratory technique for detecting proteins by antibodies. Researchers frequently use bovine serum albumin (BSA) as a blocking agent to prevent non-specific binding of antigens and antibodies to the microtiter
Tamara Topală et al.
Clujul medical (1957), 87(4), 215-219 (2014-01-01)
The continuous search for new molecules with therapeutic abilities has led to the synthesis and characterization of a large number of metal complexes, proven to exhibit potential as pharmacological agents through their antibacterial, antiviral, antifungal and antineoplastic properties. As serum



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