跳转至内容
Merck
CN

14-173-M

MAP Kinase 2/Erk2 Protein, active, mouse, 10 µg

Active, N-terminal GST-tagged, recombinant MAP Kinase 2, expressed in E. coli cells, activated using MEK1 (Catalog #14-429). For use in Kinase Assays.

登录 查看组织和合同定价。

选择尺寸

变更视图

关于此项目

UNSPSC Code:
12352202
NACRES:
NA.41
eCl@ss:
32160405
Biological source:
mouse
Recombinant:
expressed in E. coli
技术服务
需要帮助?我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助


biological source

mouse

Quality Level

recombinant

expressed in E. coli

mol wt

Mw 67.8 kDa

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable (kinase)

NCBI accession no.

UniProt accession no.

General description

N-terminal GST-tagged, recombinant MAP Kinase 2, expressed in E. coli cells; activated using MEK1 (Catalog #14-429).
Product Source: recombinant, mouse, full-length MAP Kinase 2

Biochem/physiol Actions

Protein Target: MAPK2
Target Sub-Family: CMGC

Packaging

Also available in 250 µg size --call for pricing and availability and reference catalog number 14-173M when ordering the 250 µg size.

Physical form

50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 50% glycerol, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol.
Glutathione agarose; Ni2+/NTA agarose

Preparation Note

Store in undiluted aliquots at -20°C for up to one year from the date of receipt. Avoid repeatede freeze-thaw cycles.

Analysis Note

Routinely evaluated by phosphorylation myelin basic protein

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Skin Sens. 1

存储类别

10 - Combustible liquids

wgk

WGK 2



分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库



Identification of insulin-stimulated protein kinase-1 as the rabbit equivalent of rskmo-2. Identification of two threonines phosphorylated during activation by mitogen-activated protein kinase.
Sutherland, C, et al.
European Journal of Biochemistry, 212, 581-588 (1993)
B Zhang et al.
The Journal of biological chemistry, 271(50), 31771-31774 (1996-12-13)
Peroxisome proliferator-activated receptor (PPAR) gamma plays an important role in adipocyte differentiation and the regulation of adipocyte gene expression. Insulin also serves to promote adipogenesis. We report that insulin and a PPARgamma ligand (thiazolidinedione (TZD)) stimulate in a synergistic manner
alpha7 Nicotinic Acetylcholine Receptors Mediate {beta}-Amyloid Peptide-induced Tau Protein Phosphorylation
Wang, H. Y., et al
The Journal of Biological Chemistry, 278, 31547-31553 (2003)



全球贸易项目编号

货号GTIN
14-17304053252367427
14-173M04053252362149