203390
(±)-Blebbistatin
≥97% (HPLC), solid, Myosin II inhibitor, Calbiochem®
别名:
(±)-博司他汀
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关于此项目
经验公式（希尔记法）:
C18H16N2O2
化学文摘社编号:
分子量:
292.33
MDL number:
UNSPSC Code:
12352200
NACRES:
NA.77
Assay:
≥97% (HPLC)
Form:
solid
Quality level:
Storage condition:
OK to freeze, protect from light
产品名称
(±)-Blebbistatin, (±)-Blebbistatin, CAS 674289-55-5, is a cell-permeable, selective, and reversible inhibitor of nonmuscle myosin II. Blocks cell blebbing and disrupts cell migration & cytoKinesis in vertebrate cells.
Quality Level
assay
≥97% (HPLC)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, protect from light
color
yellow
solubility
methanol: 1.5 mg/mL, 100% DMSO: 100 mg/mL, 90% DMSO: 75 mg/mL
shipped in
wet ice
storage temp.
−20°C
SMILES string
N2(CCC3(C2=Nc4c(cc(cc4)C)C3=O)O)c1ccccc1
InChI
1S/C18H16N2O2/c1-12-7-8-15-14(11-12)16(21)18(22)9-10-20(17(18)19-15)13-5-3-2-4-6-13/h2-8,11,22H,9-10H2,1H3
InChI key
LZAXPYOBKSJSEX-UHFFFAOYSA-N
General description
一种可渗透细胞的化合物，作为非肌肉肌球蛋白Ⅱ的选择性、强效和可逆抑制剂。抑制人血小板（≤100 µM）的ATP酶和滑动运动，而不影响肌球蛋白轻链激酶（MLCK）的活性。已经显示出可以阻止细胞起泡并迅速破坏脊椎动物细胞中的定向细胞迁移和胞质分裂。不会破坏有丝分裂或影响收缩环的组装。也可使用50 mM（5 mg/342 µl）（±）-Blebbistatin在90% DMSO的溶液（目录号203389）。
Biochem/physiol Actions
主要靶标
ATPase
ATPase
产物不与ATP竞争。
可逆性：是
细胞可渗透性：是
靶标IC50：<100 µM 抑制人血小板的ATP酶和滑动运动
Packaging
用惰性气体包装
Preparation Note
在90% DMSO中复溶后，等分并冷冻保存（-20°C）。90% DMSO中的储备液可在-20°C下稳定保存1个月。100%DMSO中的储备溶液不稳定；临用前复溶。
Other Notes
Shu, S., et al. 2005.Proc.Natl.Acad.Sci. USA102, 1472.
Kovacs, M., et al. 2004.J. Biol. Chem.279, 35557.
Straight, A.F., et al. 2003.Science299, 1743.
Cheung, A., et al. 2001.Mol.Biol. Cell Suppl.12, 271a.
Kovacs, M., et al. 2004.J. Biol. Chem.279, 35557.
Straight, A.F., et al. 2003.Science299, 1743.
Cheung, A., et al. 2001.Mol.Biol. Cell Suppl.12, 271a.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Tom Stiff et al.
Cell reports, 31(8), 107681-107681 (2020-05-28)
Centrosome separation in late G2/ early prophase requires precise spatial coordination that is determined by a balance of forces promoting and antagonizing separation. The major effector of centrosome separation is the kinesin Eg5. However, the identity and regulation of Eg5-antagonizing
Claire Dziengelewski et al.
The Journal of cell biology, 219(4) (2020-04-25)
The tumor cell-selective killing activity of the adenovirus type 2 early region 4 ORF4 (E4orf4) protein is poorly defined at the molecular level. Here, we show that the tumoricidal effect of E4orf4 is typified by changes in nuclear dynamics that
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| 203390-5MG | 04055977221268 |