产品名称
磷酸酶抑制剂混合物套装V,50X, Phosphatase Inhibitor Cocktail Set V is a 50X cocktail of four serine/threonine and tyrosine phosphatase inhibitors.
form
liquid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, protect from light
solubility
water: soluble
shipped in
wet ice
storage temp.
−20°C
General description
一种由四种磷酸酶抑制剂组成的混合物,可同时抑制丝氨酸/苏氨酸和蛋白酪氨酸磷酸酶。可以1 ml或一组5个1 ml小瓶的形式提供。
Application
磷酸酶抑制剂混合物套装V(50X)的应用实例:
- 加入裂解缓冲液以分离代谢活化(metabolically activated one)骨髓巨噬细胞(BMDM)用于蛋白质免疫印迹
- 加入放射性免疫沉淀(RIPA)改良缓冲液以孵育人脐静脉内皮细胞(HUVEC)培养物用于蛋白质免疫印迹
- 加入裂解缓冲液以裂解HUVEC培养物用于免疫沉淀
- 加入洗涤缓冲液以洗涤蛋白复合物
- 加入4-羟乙基哌嗪乙磺酸(HEPES)缓冲液以均质化J774细胞,用于评估脂多糖(LPS)孵育对细胞质磷脂酶A2(cPLA2)活性的影响
Biochem/physiol Actions
蛋白磷酸化和去磷酸化在信号转导中起重要作用,调控细胞分裂、增殖和凋亡等各种细胞过程。蛋白质激酶会给特定位点的丝氨酸、苏氨酸或酪氨酸残基加上磷酸基团,改变蛋白的构象及与配体相互作用的能力。接着蛋白质磷酸酶去除磷酸基团,使蛋白质恢复原本的去磷酸化状态。从细胞和组织裂解物中提取磷酸化蛋白时,保持它们的磷酸化状态至关重要。
Features and Benefits
- 方便:即用型制剂,使用便捷。
- 稳定:高品质制剂,保证可靠、可重复性能。
- 多用:该混合物靶向不同类别的磷酸酶,在实验中发挥多功能作用。
Physical form
在H₂O中。
Preparation Note
初次解冻后,分装并冷冻(-20°C)以进行长期储存。临用前按1:50稀释。
Other Notes
每小瓶含1 mL包含下列组分的水溶液:
- 250 mM氟化钠
- 50 mM β-磷酸甘油(货号35675)
- 50 mM十水焦磷酸钠
- 50 mM原钒酸钠
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
毒性:有毒(F)
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signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2
存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
商品
蛋白磷酸化是一种可逆的翻译后加工,在细胞内信号传递中有非常重要的功能。采用Western Blotting技术检测磷酸化蛋白是探讨信号传导中上游调控、下游功能调整、信息交流及反馈机制等的基本手段。我们提供专用的优质试剂和研究方法,帮助你获得准确、灵敏的磷酸化检测数据。
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| 524629-1ML | 04055977197532 |
| 524629-1SET | 04055977197549 |
