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Merck
CN

539131

Protease Inhibitor Cocktail I

lyophilized, for the inhibition of aspartic, cysteine, serine, metalloproteases and aminopeptidases

别名:

蛋白酶抑制剂混合物

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关于此项目

UNSPSC Code:
12352200
NACRES:
NA.28
Form:
lyophilized
Solubility:
water: soluble
Storage temp.:
−20°C
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产品名称

蛋白酶抑制剂混合物套装I, A cocktail of five protease inhibitors that will inhibit a broad range of proteases and esterases. Supplied with a data sheet.

form

lyophilized

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, desiccated (hygroscopic)

solubility

water: soluble

shipped in

wet ice

storage temp.

−20°C

General description

蛋白酶抑制剂混合液Set I A 是一种专门配制的混合液,含有可抑制各种蛋白酶和酯酶的五种不同蛋白酶抑制剂。

Application

蛋白酶抑制剂鸡尾酒套装I已用作以下产品的补充剂:
  • 冷放射免疫沉淀法(RIPA)缓冲液,用于制备蛋白质印迹分析中的细胞裂解液
  • RIPA裂解缓冲液,用于提取大鼠肝组织总蛋白和AML12细胞
  • 蔗糖溶液,用于均质化小鼠皮质以分离髓鞘

蛋白酶抑制剂混合液 Set I – Calbiochem 已用于裂解缓冲液中以裂解细胞并进行蛋白免疫印迹。

Biochem/physiol Actions

抑制多种蛋白酶和酯酶。
蛋白酶抑制剂鸡尾酒和特异性蛋白酶抑制剂是科学家在各种蛋白质组学研究中使用的重要工具。这些研究包括蛋白质表达、表征、生物标志物发现、翻译后修饰、蛋白质表达谱和定量蛋白质测量。蛋白酶抑制剂鸡尾酒或蛋白酶抑制剂的应用保证了蛋白质完整性的保存,便于下游分析和表征。

Features and Benefits

  • 蛋白酶抑制剂混合液保护粗提取物中的目标蛋白免于分解
  • 可立即用于重构(reconstitution)

Preparation Note

复溶后,等分并冷冻保存(-20°C)。贮备溶液在-20°C下可稳定保存至多1个月。
用1 ml dH2O复溶每个小瓶,以获得100X的贮备溶液。

Other Notes

如果直接用IMAC树脂处理细胞提取物,EDTA 的存在可能干扰His•Tag 融合蛋白的纯化。

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

毒性:有害(C)


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signalword

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Eye Dam. 1 - Skin Corr. 1A - STOT RE 2 Inhalation

target_organs

Respiratory Tract

存储类别

8B - Non-combustible corrosive hazardous materials

flash_point_f

Not applicable

flash_point_c

Not applicable



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全球贸易项目编号

货号GTIN
539131-1VL04055977195378
539131-10VL07790788051273