539134
Protease Inhibitor Cocktail III
EDTA-Free, liquid, for the inhibition of aspartic, cysteine, and serine proteases and aminopeptidases in mammalian cells and tissues.
别名:
无EDTA蛋白酶抑制剂复合物, 蛋白酶抑制剂混合物III
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关于此项目
UNSPSC Code:
12352200
NACRES:
NA.77
Form:
liquid
Solubility:
water: soluble
Storage temp.:
−20°C
产品名称
蛋白酶抑制剂混合物套装III，无EDTA, Protease inhibitor cocktail III, EDTA-free for inhibiting aspartic, cysteine, and serine proteases as well as aminopeptidases in mammalian cells and tissues.
form
liquid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
solubility
water: soluble
shipped in
wet ice
storage temp.
−20°C
General description
注意：以1 x 1 ml或一组5 x 1 ml形式提供。
该蛋白酶抑制剂混合物是由六种蛋白酶抑制剂组成的特殊混合物，具有广泛的特异性，可抑制天冬氨酸、半胱氨酸和丝氨酸蛋白酶以及氨基肽酶。建议将该混合物与哺乳动物细胞和组织提取物一起使用，但也适用于细菌提取物。它以单个试剂瓶或一组5个试剂瓶的形式提供。每个小瓶包含1 ml蛋白酶抑制剂混合物，该混合物溶于含有少量水的DMSO中。
Application
蛋白酶抑制剂混合物套装III（无EDTA）的应用实例：
- 作为组分加入CelLytic MT细胞裂解试剂或裂解缓冲液以均质化肾脏类器官用于免疫印迹分析
- 加入均质缓冲液以均质化小鼠股四头肌组织
- 加入裂解缓冲液以提取蛋白用于免疫印迹
Biochem/physiol Actions
广泛抑制丝氨酸蛋白酶、半胱氨酸蛋白酶、天冬氨酸蛋白酶以及氨基肽酶。
蛋白酶抑制剂混合物和特异性蛋白酶抑制剂在科研人员进行的各种蛋白质组研究中发挥重要作用。研究包括蛋白质表达和表征、生物标志物发现、翻译后修饰鉴定、蛋白表达谱分析和蛋白定量测定。蛋白酶抑制剂混合物或特异性蛋白酶抑制剂确保蛋白保留完整性，以备准确的下游分析和全面的样品鉴定。
Features and Benefits
- 不含EDTA：推荐用于固定化金属亲和色谱(IMAC)。
- 多用：适合处理细菌提取物、哺乳动物细胞提取物和组织提取物。
- 快速：片剂溶解无需等待。
- 经济：即用形式减少浪费。
- 高效：1 ml可处理约20 g组织。
- 全面：包含可抑制天冬氨酸、半胱氨酸、丝氨酸蛋白酶和氨基肽酶的6种蛋白酶抑制剂。
Preparation Note
初次融化后，等分并冷冻（-20°C）。
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
毒性：刺激性（B）
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Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
存储类别
10 - Combustible liquids
flash_point_f
188.6 °F - (refers to pure substance)
flash_point_c
87 °C - (refers to pure substance)
商品
Learn tips and tricks for how to properly use inhibitors including how to select the right inhibitor and how to plan experiments with inhibitors.
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| 539134-10ML | 04053252042799 |
| 539134-1ML | 07790788051280 |
| 539134-1SET | 07790788059750 |