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539480

蛋白酶 K 来源于林伯氏白色念球菌

别名:

肽链内切酶

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关于此项目

化学文摘社编号:
39450-01-6
UNSPSC Code:
12352202
NACRES:
NA.21
EC 号:
3.4.21.64 (BRENDA, IUBMB)
MDL number:
MFCD00132129
Specific activity:
≥30 mAnson units/mg dry wt, ≥40 mAnson units/mg protein
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form

lyophilized solid

Quality Level

400

specific activity

≥30 mAnson units/mg dry wt, ≥40 mAnson units/mg protein

mol wt

28.93 kDa

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

color

white

solubility

50 mM Tris-HCl, 2 mM calcium acetate, pH 8.0: soluble

foreign activity

DNase, none detected (nicking activity with pBR322, incubation for 6 h at 37°C), RNase, none detected (ribonuclease activity with MS2 RNA detected after incubation for 6 h at 37°C)

shipped in

ambient

storage temp.

2-8°C

General description

丝氨酸蛋白酶，对多种变性和天然的高分子量蛋白质具有很强的蛋白水解活性。它可使内源性核酸酶迅速蛋白水解失活，因此可用于分离mRNA和高分子量DNA。优先裂解N-取代疏水性、脂肪族和芳香族氨基酸羰基旁的键。可被DFP、Hg2+和PMSF抑制。不会被金属螯合剂、巯基试剂、TLCK或TPCK灭活。

Application

在分离DNA和RNA的过程中，对核酸酶的蛋白水解失活起作用。
在分离DNA和RNA的过程中，用于核酸酶的蛋白水解失活。
用于去除内毒素结合的阳离子蛋白，如溶菌酶和核糖核酸酶A。
据报道，其可用于分离肝、酵母和绿豆线粒体
用于测定酶在细胞膜上的定位
用于石蜡包埋组织切片的处理，暴露用于抗体标记的抗原结合位点。
用于可传播海绵状脑病(TSE)研究中朊病毒脑组织样本蛋白的消化。

Biochem/physiol Actions

蛋白酶K是一种稳定且具有高活性的丝氨酸蛋白酶。晶体和分子结构研究证据表明该酶属于枯草素家族，具有活性位点催化三联体(Asp39-His69-Ser224)。它在一系列广泛的环境中均稳定：pH，缓冲盐，去污剂（SDS）以及温度。 在0.1-0.5% SDS存在的情况下，蛋白酶K可保持活性，在不损害分离DNA完整性的前提下，可以消化DNA制剂中的多种蛋白质和核酸酶。

Physical form

由乙酸钙冻干

Preparation Note

复溶后，储存在冰箱中（4°C）。用50 mM Tris-HCl、2 mM乙酸钙制备的pH 8.0的储备溶液在4°C下可稳定保存1年。

Other Notes

Watazu, Y., et al. 1993.J. Lab. Clin. Anal.7, 81.
Lebherz, H.G., et al. 1986.生物化学。J.233, 51.
Ebeling, W., et al. 1974.Eur. J. Biochem.47, 91.
一个mAnson单位定义为在37°C、pH 7.5条件下每分钟释放1.0 µM Folin阳性氨基酸（以酪氨酸计）的酶的量。

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

毒性：刺激性（B）

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pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

存储类别

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

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全球贸易项目编号

货号GTIN
279633-5G04061826211403
841656001004022536648668
279633-25G04061826211397
539480-1GM04055977269116
539480-25MG07790788051327
539480-100MG04055977269147
539480-10GM04055977269123