540411
嘌呤霉素，二盐酸盐，经细胞培养测试
Puromycin, CAS 58-58-2, is a protein synthesis inhibitor that causes premature release of nascent polypeptide chains.
别名:
嘌呤霉素，二盐酸盐，经细胞培养测试
登录 查看组织和合同定价。
选择尺寸
变更视图
关于此项目
Quality Level
assay
≥98% (HPLC)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, desiccated
impurities
≤10 EU/mg Endotoxin (dry weight)
color
white to off-white
solubility
methanol: 20 mg/mL, water: 50 mg/mL
shipped in
ambient
storage temp.
−20°C
InChI
1S/C22H29N7O5.2ClH/c1-28(2)19-17-20(25-10-24-19)29(11-26-17)22-18(31)16(15(9-30)34-22)27-21(32)14(23)8-12-4-6-13(33-3)7-5-12;;/h4-7,10-11,14-16,18,22,30-31H,8-9,23H2,1-3H3,(H,27,32);2*1H/t14-,15+,16?,18+,22+;;/m0../s1
InChI key
MKSVFGKWZLUTTO-USYAMCSGSA-N
General description
一种氨基核苷类抗生素，可作为原核和真核蛋白质合成抑制剂。类似于氨酰tRNA的氨酰腺苷酸末端，竞争与大核糖体亚单位的“A位点”结合。其掺入生长中的多肽，导致链延伸终止和新生多肽基嘌呤霉素的释放。用于选择表达转染pac基因（白链霉菌）的哺乳动物细胞系，其产物嘌呤霉素乙酰转移酶通过乙酰化使药物失活。诱导胸腺细胞和HL-60白血病细胞内DNA断裂。转染哺乳动物细胞的推荐选择范围为1至30 µg/ml。
嘌呤霉素，CAS 58-58-2，是一种蛋白质合成抑制剂，可导致新生多肽链过早释放。
蛋白质合成抑制剂。通过导致新生多肽链过早释放来抑制翻译。诱导胸腺细胞和HL-60白血病细胞内DNA断裂。
Biochem/physiol Actions
首要靶标
蛋白质合成
蛋白质合成
Preparation Note
复溶后，通过0.22 µm孔径的过滤器过滤灭菌，等分并冷冻（-20°C）。贮备溶液在-20°C下可稳定保存至多3个月。
Other Notes
Chow, S.C., et al. 1995.Exp.Cell Res.216, 149.
Kaufman, S.H., et al. 1993.Cancer Res.53, 3976.
de la Luna, S. and Ortín, J. 1992.Methods Enzymol.216, 376.
Chow, S.C., et al. 1991.Exp.Cell Res. 216, 149.
Kaufman, S.H., et al. 1993.Cancer Res.53, 3976.
de la Luna, S. and Ortín, J. 1992.Methods Enzymol.216, 376.
Chow, S.C., et al. 1991.Exp.Cell Res. 216, 149.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
毒性：有害（C）
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
涉药品监管产品
此项目有
Amy L Pashler et al.
RNA (New York, N.Y.), 27(10), 1265-1280 (2021-07-17)
XRN1 is a highly conserved exoribonuclease which degrades uncapped RNAs in a 5'-3' direction. Degradation of RNAs by XRN1 is important in many cellular and developmental processes and is relevant to human disease. Studies in D. melanogaster demonstrate that XRN1
Ah-Ram Kim et al.
Current protocols, 2(12), e628-e628 (2022-12-27)
Nanobodies have emerged as powerful protein-binding tools to uncover protein functions. Using functionalized protein binders, proteins of interest can be visualized, degraded, delocalized, or post-translationally modified in vivo. We recently reported the use of two short peptide tags, 10-aa 127D01
Young Jin Kim et al.
Proceedings of the National Academy of Sciences of the United States of America, 119(3) (2022-01-13)
Mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene cause cystic fibrosis (CF), and the CFTR-W1282X nonsense mutation causes a severe form of CF. Although Trikafta and other CFTR-modulation therapies benefit most CF patients, targeted therapy for patients with
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| 540411-25MG | 04055977268805 |
| 540411-100MG | 07790788051402 |