biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized
does not contain
preservative
species reactivity
human, rat, mouse, bovine
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
dilution
(ELISA (1:2500-1:10,000)
Frozen Sections
Immunoblotting (1:200)
Immunocytochemistry (1:500-1:2500)
Immunoprecipitation (1 µg/200 µg lysate))
isotype
IgG
shipped in
ambient
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
mouse ... Syn1(20964)
General description
免疫亲和纯化的兔多克隆抗体。识别突触I蛋白。
识别大鼠大脑中的突触I蛋白。
这种抗突触蛋白I兔pAb经验证可用于 ELISA、免疫印迹、免疫细胞化学、免疫沉淀,以检测突触蛋白I。
Immunogen
牛
纯化的牛脑突触素I
Application
ELISA(1:2500-1:10000)
冰冻切片(见应用参考文献)
免疫印迹(1:200)
免疫细胞化学(1:500-1:2500)
免疫沉淀(1 µg/200 µg裂解物)
冰冻切片(见应用参考文献)
免疫印迹(1:200)
免疫细胞化学(1:500-1:2500)
免疫沉淀(1 µg/200 µg裂解物)
Physical form
从5 mM碳酸氢铵中冻干。
Preparation Note
用50µl PBS复溶。复溶后,等分并冷冻保存(-20°C)。避免溶液反复冻融。
Analysis Note
大鼠脑
阳性对照
阳性对照
Other Notes
Stone, L.M., et al. 1994.J. Neurosci.14, 301.
Mandell, J.W., et al. 1992.J. Neurosci.12, 1736.
Fletcher, T.L., et al. 1991.J. Neurosci.11, 1617.
Moore, R.Y., and Berstein, M. 1989.J. Neurosci.9, 2151.
Mandell, J.W., et al. 1992.J. Neurosci.12, 1736.
Fletcher, T.L., et al. 1991.J. Neurosci.11, 1617.
Moore, R.Y., and Berstein, M. 1989.J. Neurosci.9, 2151.
该抗体对突触蛋白I具有特异性,可用于估计突触密度和突触发生。在1:200稀释的免疫印迹中可以看到突触蛋白I双峰的强而特异的免疫标记。这种免疫标记可以通过抗体与突触蛋白I的预吸附来阻止。与测定条件相关的变量将决定适当的工作稀释度。
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
毒性:标准处理(A)
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存储类别
11 - Combustible Solids
wgk
WGK 1
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The Journal of neuroscience : the official journal of the Society for Neuroscience, 27(10), 2513-2524 (2007-03-09)
Collapsing response mediator protein-1 (CRMP-1) was initially identified in brain and has been implicated in plexin-dependent neuronal function. The high amino acid sequence identity among the five CRMPs has hindered determination of the functions of each individual CRMP. We generated
Alexandra Grubman et al.
Stem cell reports, 14(6), 1018-1032 (2020-05-23)
Multiple protocols have been published for generation of iMGLs from hESCs/iPSCs. To date, there are no guides to assist researchers to determine the most appropriate methodology for microglial studies. To establish a framework to facilitate future microglial studies, we first
Priya Srikanth et al.
Translational psychiatry, 8(1), 245-245 (2018-11-10)
The identification of convergent phenotypes in different models of psychiatric illness highlights robust phenotypes that are more likely to be implicated in disease pathophysiology. Here, we utilize human iPSCs harboring distinct mutations in DISC1 that have been found in families
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| 574777-10UG | 04055977189735 |