688000
Y-27632
≥95% (HPLC), solid, Rho kinase inhibitor, Calbiochem
别名:
Y-27632, ROCK抑制剂，(R)-(+)-反式-N-(4-吡啶基)-4-(1-氨基乙基)-环己烷甲酰胺，2HCl，Rho激酶抑制剂VI
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关于此项目
经验公式（希尔记法）:
C14H21N3O · 2HCl · xH2O
化学文摘社编号:
分子量:
320.26 (anhydrous basis)
MDL number:
UNSPSC Code:
12352200
NACRES:
NA.77
Assay:
≥95% (HPLC)
Form:
solid
Quality level:
Storage condition:
OK to freeze, desiccated (hygroscopic), protect from light
产品名称
Y-27632-CAS 331752-47-7-Calbiochem, Y-27632A, CAS 331752-47-7, is a cell-permeable, reversible, inhibitor of Rho kinases (Ki = 140 nM for p160ROCK). Enhances survival & cloning efficiency of ESC without affecting their pluripotency.
Quality Level
assay
≥95% (HPLC)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, desiccated (hygroscopic), protect from light
color
white
solubility
water: 100 mg/mL
shipped in
ambient
storage temp.
−20°C
SMILES string
N[C@@H](C1CCC(CC1)C(=O)Nc2ccncc2)C
InChI
1S/C14H21N3O/c1-10(15)11-2-4-12(5-3-11)14(18)17-13-6-8-16-9-7-13/h6-12H,2-5,15H2,1H3,(H,16,17,18)/t10-,11?,12?/m1/s1
InChI key
IYOZTVGMEWJPKR-VOMCLLRMSA-N
General description
Rho相关蛋白激酶的一种高效、细胞可渗透性、可逆且选择性抑制剂（针对p160ROCK的Ki= 140 nM）。还可同等高效的抑制ROCK-II。这种抑制对于ATP具有竞争性。比p160ROCKε有10至50倍更低的PKC亲和力。即使在更高的浓度（~100 µM）下也不影响p21激活蛋白激酶（PAK）的活性。还可作为肌球蛋白磷酸化和平滑肌收缩的激动剂诱导的Ca2+敏化的一种有效抑制剂。阻止凋亡并提高解离的hES细胞的存活率和克隆效率，而不会影响其多能性。用Y-27632预处理可降低脓毒症大鼠模型中肺损伤的风险。另提供Y-27632固体的5 mM(500 µg/296 µl)H2O溶液（货号688001）和10 mM的DMSO溶液（货号688002）。
Biochem/physiol Actions
产物与ATP竞争。
可逆性：是
细胞可渗透性：具有
靶标Ki：对p160 Rho相关激酶（ROCK）为140 nM
首要靶标
p160 Rho相关蛋白激酶（ROCK）
p160 Rho相关蛋白激酶（ROCK）
Packaging
用惰性气体包装
Preparation Note
溶解后，等分并冷冻保存（-20°C）。储备液可在-20°C稳定6个月。
Other Notes
Cinel, I., et al. 2012.J. Thorac .Dis.4, 30.
Watanabe, K., et al. 2007.Nature Biotech.25, 681.
Chitaley, K., et al. 2001.Nat. Med.7, 119.
Davies, S.P., et al. 2000.生物化学。J. 351, 95.
Narumiya, S., et al. 2000.Methods Enzymol.325, 273.
Hirose, M., et al. 1998.J. Cell Biol.141, 1625.
Maekawa, M., et al. 1999.Science285, 895.
Uehata, M., et al. 1997.Nature389, 990.
Watanabe, K., et al. 2007.Nature Biotech.25, 681.
Chitaley, K., et al. 2001.Nat. Med.7, 119.
Davies, S.P., et al. 2000.生物化学。J. 351, 95.
Narumiya, S., et al. 2000.Methods Enzymol.325, 273.
Hirose, M., et al. 1998.J. Cell Biol.141, 1625.
Maekawa, M., et al. 1999.Science285, 895.
Uehata, M., et al. 1997.Nature389, 990.
Legal Information
在PCT Application W098/06，433A1的许可和三菱制药田边分公司的许可下进行销售。
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
毒性：有害（C）
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Eun-Mi Kim et al.
Methods in molecular biology (Clifton, N.J.), 651, 89-101 (2010-08-06)
The ability of embryonic stem cells (ES) cells to form cells and tissues from all three germ layers can be exploited to generate cells that can be used to treat diseases. In particular, successful generation of hematopoietic cells from ES
Shahnaz Babaei-Abraki et al.
The Journal of biological chemistry, 298(5), 101855-101855 (2022-03-27)
Human embryonic stem cells (hESCs) are vulnerable to cell death upon dissociation. Thus, dissociation is an obstacle in culturing, maintaining, and differentiating of hESCs. To date, apoptosis has become the focus of research into the nature of cell death triggered
Jessica L Teo et al.
Molecular biology of the cell, 31(23), 2557-2569 (2020-09-10)
Epithelia can eliminate apoptotic cells by apical extrusion. This is a complex morphogenetic event where expulsion of the apoptotic cell is accompanied by rearrangement of its immediate neighbors to form a rosette. A key mechanism for extrusion is constriction of
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| 688000-100MG | 04055977225402 |
| 688000-1MG | 07790788052362 |
| 688000-10MG | 04055977225396 |
| 688000-5MG | 07790788052379 |