跳转至内容
Merck
CN

70954

Rosetta(DE3)感受态细胞 - Novagen

Escherichia coli, rod shaped

别名:

BL21 derivatives

登录 查看组织和合同定价。

选择尺寸

变更视图

关于此项目

UNSPSC Code:
41106202
NACRES:
NA.81
Biological source:
Escherichia coli
Growth mode:
adherent or suspension
Morphology:
rod shaped
Manufacturer/tradename:
Novagen®
技术服务
需要帮助?我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助


产品名称

Rosetta(DE3)感受态细胞 - Novagen, Rosetta host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli.

biological source

Escherichia coli

Quality Level

manufacturer/tradename

Novagen®

storage condition

OK to freeze

growth mode

adherent or suspension

morphology

rod shaped

technique(s)

microbiological culture: suitable

shipped in

wet ice

storage temp.

−70°C

General description

Rosetta宿主菌株是BL21衍生物,旨在增强真核蛋白的表达,该蛋白含有在大肠杆菌中很少使用的密码子。这些菌株在兼容的氯霉素抗性质粒上为AGG、AGA、AUA、CUA、CCC、GGA密码子提供tRNA。因此,Rosetta菌株提供了“通用”翻译,否则该翻译受大肠杆菌密码子使用的限制。tRNA基因由其天然启动子驱动。在Rosetta(DE3)pLysS中,稀有tRNA基因存在于携带T7溶菌酶基因的相同质粒上。

DE3表明宿主是λDE3的溶原菌,因此在lacUV5启动子的控制下携带T7 RNA聚合酶基因的染色体拷贝。这样的菌株适用于通过用IPTG诱导从克隆在pET载体中的靶基因生产蛋白质。
基因型:F-ompT hsdSB(rB- mB-) gal dcm (DE3) pRARE (CamR)

该产品包含转基因生物(GMO)。在欧盟范围内,转基因物种分别受欧洲议会和理事会的2001/18 /EC和2009/41/EC指令及其成员国中国家所实施规范的约束。该法规规定我们有义务索取您以及处理转基因生物的机构的相关信息。请点击这里获得最终用户声明(EUD)表格。
Rosetta宿主菌株是BL21衍生物,旨在增强真核蛋白的表达,该蛋白含有在大肠杆菌中很少使用的密码子。

Other Notes

0.4 ml1 ml组分

•2 × 0.2 ml5 × 0.2 mlRosetta(DE3)感受态细胞

•2 × 2 ml4 × 2 mlSOC培养基

•2 ng2 ng供试质粒

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

毒性:多个毒性值,请参阅MSDS(O)


存储类别

10 - Combustible liquids

wgk

WGK 2



分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库



Gilberto Betancor et al.
Cell reports, 29(7), 1923-1933 (2019-11-14)
Myxovirus resistance 2 (MX2/MXB) is an interferon (IFN)-induced HIV-1 restriction factor that inhibits viral nuclear DNA accumulation. The amino-terminal domain of MX2 binds the viral capsid and is essential for inhibition. Using in vitro assembled Capsid-Nucleocapsid (CANC) complexes as a surrogate
Alireza Edraki et al.
Molecular cell, 73(4), 714-726 (2018-12-26)
CRISPR-Cas9 genome editing has transformed biotechnology and therapeutics. However, in vivo applications of some Cas9s are hindered by large size (limiting delivery by adeno-associated virus [AAV] vectors), off-target editing, or complex protospacer-adjacent motifs (PAMs) that restrict the density of recognition sequences
Monika I Mayr et al.
PloS one, 6(11), e27471-e27471 (2011-11-22)
Members of the kinesin-8 subfamily are plus end-directed molecular motors that accumulate at the plus-ends of kinetochore-microtubules (kt-MTs) where they regulate MT dynamics. Loss of vertebrate kinesin-8 function induces hyperstable MTs and elongated mitotic spindles accompanied by severe chromosome congression



全球贸易项目编号

货号GTIN
70954-307790788052904
70954-407790788052911