Anti-Dopamine β Hydroxylase Antibody, NT

Epitope: N-terminus

Synthetic peptide, CSAPRESPLPYHIPLDPEG-amide, cloned from human (DbetaH) cDNA (Nagatsu et al. 1990).

Detect Dopamine β Hydroxylase using this Anti-Dopamine β Hydroxylase Antibody, N-terminus validated for use in IH & WB.

Immunoblotting: 1:250-1:1,000.

Immunohistochemistry: 1:250-1:1,000, staining was evaluated in the locus coeruleus of formaldehyde-fixed, cryopreserved human and monkey brain Showed weak staining on both human and monkey.

Optimal working dilutions must be determined by the end user.

Research Category
Neuroscience

Research Sub Category
Neurotransmitters & Receptors

Neuronal & Glial Markers

Dopamine Beta Hydroxylase (DbetaH) N-terminal. By immunoblot the antibody specifically reacts with a single (or double) band(s) of M.W. »70-75,000 in samples of SDS-solubilized, DTT-reduced human adrenal medulla subjected to SDS-PAGE.

Liquid in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

Maintain at -20 to -70°C in undiluted aliquots for up to six months. Avoid repeated freeze-thaw cycles.

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