Anti-Adenosine A3 Receptor Antibody, extracellular (a.a. 154-168)

Amino acid sequence corresponding to the 3rd extracellular domain (15 amino acids) of the rat A3R gene.

Epitope: extracellular (a.a. 154-168)

Anti-Adenosine A3 Receptor Antibody, extracellular (a.a. 154-168) is an antibody against Adenosine A3 Receptor for use in ELISA, IC & WB.

Research Category
Neuroscience

Research Sub Category
Neurotransmitters & Receptors

Circadian Rhythm & Sleep

Western blot: 1-10 micrograms per mL: A3 receptors are different sizes in different species & tissues; A3R from rat brain membranes runs as a 40kDa band, 52kDa in rabbit uroepithelium membranes (Yu, W et al. Am J. Physiol Cell Physiol 2006 in press); use proteinase inhibitors and membrane preparations for best signals.

ELISA: 1:10,000-1:50,000 (50-100 ng A3R peptide (Catalog Number AG294)/well)

Immunocytochemistry: Caufield, 2003. Light 2% PFA fixation 5-10 minutes --long fixations lower staining intensity.

Optimal working dilutions must be determined by end user.

Specific for Adenosine A3 receptor. Based on mRNA studies, the A3R has been found in rat testis and lung, kidneys, heart and brain. Other studies have found the mRNA to be highest in lung, spleen and pineal glands and moderate levels in brain kidney and testis.

Affinity purified immunoglobulin. Liquid in PBS with 0.1% BSA

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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