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Merck
CN

AB5204

抗钠通道抗体,电压门控,脑I型

Chemicon®, from rabbit

别名:

Nav1.1, SCN1A

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IHC, WB
Citations:
19
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biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, rat

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable, western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... SCN1A(6323)

Immunogen

来自大鼠I型电压门控钠通道(VGSC)的α亚基465-481的纯化肽(登录号P04774)。

Application

将要接收全长蛋白的全部程序应在4C下进行,并应使用以下蛋白酶抑制剂混合物:胃蛋白酶抑制剂A(1 μg/mL),亮抑蛋白酶肽(1 μg/mL),抑肽酶(1 μg/mL),Pefabloc SC(0.2 mM),苯甲脒(0.1 mg/mL)和钙蛋白酶抑制剂I和II(各8 μg/mL)。

蛋白质印迹:在大鼠脑膜上使用ECL为L1:200。

大鼠脑固定冰冻切片和小鼠心脏组织上的免疫组化。

应使用载体蛋白(例如BSA(1-3%))进行稀释。

最佳工作稀释度必须由最终用户确定。
该抗钠通道抗体,电压门控,脑I型经验证可用于IH,WB检测钠通道。

Biochem/physiol Actions

识别VGSC的I型α亚基。不与迄今为止检测的任何其他钠通道抗原发生交叉反应。

物种反应性: 由于序列同源性,预计该抗体也与人反应。其他物种尚未经过测试。

Other Notes

浓度:请参考批次特异性浓缩物的分析证书。

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany


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hcodes

Hazard Classifications

Aquatic Chronic 3

存储类别

11 - Combustible Solids

wgk

WGK 3



分析证书(COA)

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R Planells-Cases et al.
Biophysical journal, 78(6), 2878-2891 (2000-05-29)
Neural activity is crucial for cell survival and fine patterning of neuronal connectivity during neurodevelopment. To investigate the role in vivo of sodium channels (NaCh) in these processes, we generated knockout mice deficient in brain NaChalpha(II). NaChalpha(II)(-/-) mice were morphologically
Persistent Nav1.6 current at axon initial segments tunes spike timing of cerebellar granule cells.
Nancy Osorio,Laurence Cathala,Miriam H Meisler,Marcel Crest,Jacopo Magistretti,Patrick Delmas
The Journal of Physiology null
D Zhou et al.
The Journal of cell biology, 143(5), 1295-1304 (1998-12-02)
Voltage-gated sodium channels (NaCh) are colocalized with isoforms of the membrane-skeletal protein ankyrinG at axon initial segments, nodes of Ranvier, and postsynaptic folds of the mammalian neuromuscular junction. The role of ankyrinG in directing NaCh localization to axon initial segments



全球贸易项目编号

货号GTIN
AB520404053252323881
AB5204-50UL04053252334955